| 引用本文： |
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李艳苓,王勤,陈勇.高效液相色谱法测定三叶香茶菜中齐墩果酸含量[J].广西科学院学报,2006,22(S1):406-407. [点击复制]
- LI Yan-ling,WANG Qin,CHEN Yong.Determination of Oleandic Acid in Herba Isodonis Ternifoliae by HPLC[J].Journal of Guangxi Academy of Sciences,2006,22(S1):406-407. [点击复制]
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| 摘要: |
| 采用高效液相色谱(HPLC)法,选用大连依利特Hypersil ODS2色谱柱,流动相甲醇-0.1%磷酸溶液(80:20),检测波长207nm,柱温22℃,测定广西三叶香茶菜中的齐墩果酸含量。结果表明,齐墩果酸在0.175~7μg之间呈线性关系,齐墩果酸平均回收率为98.91%,RSD为1.886%。该方法准确可行,重复性好,可以作为测定三叶香茶菜中齐墩果酸含量的方法。 |
| 关键词: 高效液相色谱法 齐墩果酸 三叶香茶菜 |
| DOI： |
| 投稿时间：2006-08-20 |
| 基金项目:广西科技厅科技攻关项目(桂科攻0537025-3B) |
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| Determination of Oleandic Acid in Herba Isodonis Ternifoliae by HPLC |
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LI Yan-ling, WANG Qin, CHEN Yong
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| (Medicinal College, Guangxi Traditional Chinese Medical University, Nanning, Guangxi, 530001, China) |
| Abstract: |
| A HPLC method was developed.The Elite Hypersil ODS2 column was used,and mobile phase was composed of CH3OH-0.1%H3PO4 solution(80:20),the detection wavelength was at 207 nm and the column temperature was 22℃.The result showed that oleandic acid had the linear range of 0.1757μg,the average recovery of oleandic acid was 98.91%,RSD=1.886%.The method of quantification is accurate,realiable and reproducible.It can be used effectively for the HPLC determination of oleandic acid in Herba isodonis ternifoliae. |
| Key words: HPLC oleandic acid herba isodonis ternifoliae |
