| 引用本文： |
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莫建光,周琳,陈秋虹,卢安根.高效液相色谱法测定保健食品中维生素B12的含量[J].广西科学院学报,2006,22(S1):408-410. [点击复制]
- MO Jian-guang,ZHOU Lin,CHEN Qiu-hong,LU An-gen.Determination of Vitamin B12 in Health Care Food by Reversed Phase High Performance Liquid Chromatography[J].Journal of Guangxi Academy of Sciences,2006,22(S1):408-410. [点击复制]
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| 摘要: |
| 采用高效液相色谱法测定保健食品中维生素B12含量,色谱柱用Agilent ZORBAX SB-Aq(4.6mm×250mm,5μm),流动相为甲醇-0.2%磷酸水溶液(38:62),流速1.0ml/min,检测波长361nm。结果是维生素B12线性范围为0.816~4.08μg,r=0.9997;平均回收率为97.2%,RSD为1.39%(n=6),最小检出限为0.85μg/g。高效液相色谱法灵敏度高,操作简便,重现性好,结果准确,可以用于保健食品中维生素B12的质量控制。 |
| 关键词: 高效液相色谱法 维生素B12 含量测定 保健食品 |
| DOI： |
| 投稿时间：2006-08-06 |
| 基金项目: |
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| Determination of Vitamin B12 in Health Care Food by Reversed Phase High Performance Liquid Chromatography |
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MO Jian-guang, ZHOU Lin, CHEN Qiu-hong, LU An-gen
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| (Guangxi Research Center for Analysis and Test, Nanning, Guangxi, 530022, China) |
| Abstract: |
| A method for detemination of Vitamin B12 heath care food by HPLC was created.The method contained a Agilent ZORBAX SB-Aq(4.6×25nm,5μm) column,with methanol-0.2%Phesphoric Acid(38:62) as mobile phase,rate 1.0ml/min,detection wavelength at 361nm.Linarity range:0.1864.08μg,r=0.9997,the average recovery of Vitamin B12 was 97.2%,the RSD of results was 1.39%(n=6),minimal detection value 0.85%μg/g.The method is sensitive,simple,precise and accurate.This study provides a test method for the quality control of health care food. |
| Key words: HPLC vitamin B12 determination health care food |
