| 引用本文： |
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周琳,莫建光,黄岛平,陈秋虹,陈玉君.高效液相色谱法测定黄疸患婴血清中维生素B2[J].广西科学院学报,2006,22(S1):411-412,415. [点击复制]
- ZHOU Lin,MO Jian-guang,HUANG Dao-ping,CHEN Qiu-hong,CHEN Yujun.Determination of Vitamin B2 in the Serum of Icterus Diseased Infant by the High Performance Liquid Chromatography[J].Journal of Guangxi Academy of Sciences,2006,22(S1):411-412,415. [点击复制]
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| 摘要: |
| 采用Oasis C18小柱吸附富集血清中的维生素B2,除去蛋白质等杂质的前处理手段,以Phenomenex Luna C18柱为分析柱、甲醇:0.02mol/L乙酸铵为3:7为流动相,荧光检测器(激发波长425nm,发射波长525nm)测定维生素B2含量。方法的平均回收率为98.0%,RSD为1.2%,最低检出浓度为0.8ng/ml。本方法操作简便,能快速、准确地检测人体血清中维生素B2含量。 |
| 关键词: 高效液相色谱法 维生素B2 血清 |
| DOI： |
| 投稿时间：2006-08-22 |
| 基金项目: |
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| Determination of Vitamin B2 in the Serum of Icterus Diseased Infant by the High Performance Liquid Chromatography |
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ZHOU Lin1, MO Jian-guang1, HUANG Dao-ping1, CHEN Qiu-hong1, CHEN Yujun2
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| (1.Guangxi Research Center for Anlysis and Test, Nanning, Guangxi, 530022, China;2.Guangxi Nanning Railway Hospital, Nanning, Guangxi, 530001, China) |
| Abstract: |
| The article created a method for determination of VitB2 in serum of icterus diseased infant by HPLC,Before the determination,absorb pregnant VitB2 in serum through the Oasis C18 column,removing the impurity of hemocyanin and so on.Taking the phenomenex Luna C18 as the analytical column,the methanol:0.02mol/L aminum actetate=3:7 as the mobile phase,the fluoresce detector(Excitation wavelength was 425 nm;Emission wavelength was 525nm).The method avergage recovery is 98.0%,RSD=1.2%,and the lowest certified concentration is 0.8ng/ml.The method is simple,rapid and accurate in determining VitB2 in serum of human body. |
| Key words: HPLC VitB2 serum |
