| 引用本文： |
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甄汉深,何翠薇,陈勇,周吴萍,苏春妹,陆晖.高效液相色谱法测定苦玄参药材中的苦玄参苷ⅠA含量[J].广西科学院学报,2006,22(S1):403-405. [点击复制]
- ZHEN Han-shen,HE Cui-wei,CHEN Yong,ZHOU Wu-ping,SU Chun-mei,LU Hui.Determination of Piefeltarraenin Ⅰ A Extracted from Picria fel-terrae Lour. by HPLC[J].Journal of Guangxi Academy of Sciences,2006,22(S1):403-405. [点击复制]
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| 摘要: |
| 采用Agilent ZORBAX Eclipse XDB C8色谱柱(4.6×150mm,5μm),以乙腈-0.5%醋酸(32:68)为流动相,流速1ml.min-1,检测波长264nm,建立高效液相色谱法(HPLC),测定广西梧州大坡种植基地和龙州县种植的苦玄参药材的茎、叶中苦玄参苷ⅠA的含量。实验结果显示,苦玄参苷ⅠA在2.28~11.4μg范围内线性良好,平均加样回收率为101.1%RSD=2.4%(n=5),梧州大坡种植基地和龙州县种植的苦玄参药材中苦玄参苷ⅠA在茎、叶中的平均含量分别为0.10%,0.31%,0.11%,0.58%。本方法简便、快速,重复性好,可实际用于苦玄参药材中苦玄参苷ⅠA含量测定。 |
| 关键词: 高效液相色谱法 苦玄参 苦玄参苷ⅠA 含量测定 |
| DOI： |
| 投稿时间：2006-03-18 |
| 基金项目:国家中医药管理局课题民族项目(04-05ZM01);广西科技厅攻关项目(桂科攻0235022-4);广西教育厅课题(桂教科研[2003]22号) |
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| Determination of Piefeltarraenin Ⅰ A Extracted from Picria fel-terrae Lour. by HPLC |
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ZHEN Han-shen, HE Cui-wei, CHEN Yong, ZHOU Wu-ping, SU Chun-mei, LU Hui
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| (Guangxi Traditional Chinese Medical University, Nanning, Guangxi, 530001, China) |
| Abstract: |
| Using the Agilent ZORBAX Eclipse XDS C8 column(4.6×150mm,5μm),with the mobile phase acetonitrile-0.5% acid(32:68),determined by 264nm ultraviolet,1ml/min flow rate,to quantify the main constituent Piefeltrraenin ⅠA in the stems and leaves of Picria fel-terrae Lour.,which were planted in Wuzhou,Dapo and Longsheng of Guangxi.The result showed that linearity of Piefeltarraenin ⅠA was good in the range of 2.2811.4μg,the recoveries of Piefeltarraenin ⅠA were 101.1%,respectively with corresponding RSD=2.4%(n=5).The main constituent Piefeltarraenin ⅠA in Picria fel-terrae Lour.in two places were 0.10%,0.31%,0.11%,0.58%.The HPLC method has the characteristics of time saving,convenience,good repeatability which can be used for quantifying the main constituent Piefeltarraenin ⅠA in Picria fel-terrae Lour. |
| Key words: high performance liquid chromatography Picria fel-terrae Lour. Piefeltarraenin ⅠA content determination |
