| 引用本文： |
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刘明团,王树声,朱田风,谢振国,李秀芳,韦一知,杨春义.小鼠诱导转基因传代细胞对AFP粪便标本中脊灰和其他肠道病毒的分离鉴别研究[J].广西科学,1997,4(2):147-149. [点击复制]
- Liu Mingtuan,Wang Shusheng,Zhu Tianfeng,Xie Zhenguo,Li Xiufang,Wei Yizhi,Yang Chunyi.Sensitivity Comparison of Lα,RD and Hep-2 Cells in Virus Isolation and Identification from Stool Specimens of AFP Cases[J].Guangxi Sciences,1997,4(2):147-149. [点击复制]
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| 摘要: |
| 报告46份AFP粪便标本用Lα、RD和Hep-2三种细胞作病毒分离,阳性率分别为26.09%、60.87%、43.48%。同步分离出脊灰病毒Ⅰ型3株,Ⅱ型2株,Ⅲ型4株,Ⅰ+Ⅱ型1株,Ⅰ+Ⅲ型1株,混合P2+E1株。脊灰病毒总分离阳性率为28.95%。非脊灰肠道病毒分离阳性率分别为0%、36.96%、23.91%。从6份第1次分离结果阴性的高危病例和高度临床病例粪便标本重新分离出1株Ⅲ型脊灰病毒和3株非脊灰肠道病毒。应用Lα细胞不仅能作型别鉴定,而且能区别脊灰病毒和非脊灰肠道病毒混合感染。 |
| 关键词: Lα RB Hep-2 细胞 病毒分离鉴定 |
| DOI： |
| 投稿时间：1997-01-15 |
| 基金项目:日本东京国立预防医学卫生研究院。 |
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| Sensitivity Comparison of Lα,RD and Hep-2 Cells in Virus Isolation and Identification from Stool Specimens of AFP Cases |
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Liu Mingtuan, Wang Shusheng, Zhu Tianfeng, Xie Zhenguo, Li Xiufang, Wei Yizhi, Yang Chunyi
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| (Guangxi Health and Anti epidemic Center, Taoyuanlu, Nanning, Guangxi, 530021) |
| Abstract: |
| Viruses were isolated simultaneously from stool specimens of 46 AFP cases by Lα,RD and Hep2 Cells,with positive rate 26.09%,60.87% and 43.48% respectively.A total of 3 strains of poliovirus Ⅰ, 2 strains of poliovirus Ⅱ,4 strains of poliovirus Ⅲ, 1 strain of poliovirus Ⅰ+Ⅱ, 1 strain of poliovirus Ⅰ+Ⅲ and 1 strain of poliovirus Ⅱ+non polio enterovirus were isolated from the specimens.The total isolation rate of poliovirus was 28.95% whereas the isolation rate of non-polio enterovirus was 0%,3696% and 23.91% respectively in the 3 kinds of cells.One strain of poliovirus Ⅲ and 3 strains of non-polio enterovirus were re-isolated by 22nd generation RD and Hep-2 cells simultaneously from specimens of high risk cases or clinical cases which were negative in the first isolation by 212th generation Hep-2 or RD cells.The application of Lα cell can not only identify the type of poliovirus,but also distinguish poliovirus infection from non-polio enterovirus infection. |
| Key words: AFP Lα cell RD cell Hep-2 cell enterovirus |
