| 引用本文： |
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苏建家,王宇,杨春,黄定瑞,冈本宏明,严瑞琪.人乙肝病毒DNA在感染树鼩肝细胞内状态的研究[J].广西科学,1997,4(2):139-142. [点击复制]
- Su Jianjia,Wang Yu,Yang Chun,Huang Dingrui,Gangben Hongming,Yan Ruiqi.The Status' Study of HHBV DNA in Liver Cells of the Infected Tree Shrews[J].Guangxi Sciences,1997,4(2):139-142. [点击复制]
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| 摘要: |
| 为探讨人乙肝病毒(HHBV)DNA在树鼩肝细胞内存在状态,对经血清学证明已被感染的树鼩肝组织进行转移印迹杂交分析。结果,在实验感染早期的树鼩肝细胞检出HBVDNA复制的中间体;在实验中晚期的动物肝细胞发现整合型的HBVDNA。表明树鼩肝对HHBV敏感,可成为乙肝和肝细胞癌发病机理研究及治疗乙肝药物筛选的较好的实验动物模型。 |
| 关键词: HBV DNA 树鼩 印迹杂交 整合 |
| DOI： |
| 投稿时间：1997-02-17修订日期：1997-04-08 |
| 基金项目:广西区卫生厅重点课题。 |
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| The Status' Study of HHBV DNA in Liver Cells of the Infected Tree Shrews |
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Su Jianjia, Wang Yu, Yang Chun, Huang Dingrui, Gangben Hongming, Yan Ruiqi
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| (Guangxi Cancer Institute, 6 Binhulu, Nanning, Guangxi, 530021) |
| Abstract: |
| The human hepatitis B virus (HHBV) markers in the serum of the experimental tree shrews that had been inoculated with HHBV were detected by radioimmunoassay (RIA) and enzyme linked immunosorbent assay (ELISA).The intermediate form of HHBV replication and integrated HHBV DNA were found in the liver cells of these tree shrews in the early and later experimental stages respectively by southern blot analysis.These results indicated that tree shrews may easily be infected with HHBV and it is a useful animal model to study the drugs for prevention and treatment of hepatitis B and the mechanism of hepatocellular development. |
| Key words: Human hepatitis B Virus Southern blot tree shrew replication intergrated |
