| 引用本文： |
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吉春喜,王炫,陆君铭,申乃坤,张红岩.耐镉菌株Cellulosimicrobium cellulans C1的筛选、鉴定及其对水稻镉污染的缓解效应[J].广西科学院学报,2026,42(2):133-142. [点击复制]
- JI Chunxi,WANG Xuan,LU Junming,SHEN Naikun,ZHANG Hongyan.Isolation,Identification of Cadmium-tolerant Strain Cellulosimicrobium cellulans C1 and Its Alleviating Effect on Cadmium Contamination in Rice[J].Journal of Guangxi Academy of Sciences,2026,42(2):133-142. [点击复制]
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| 摘要: |
| 挖掘适用于农田镉(Cadmium，Cd)污染修复的高耐Cd功能菌株，可明确其对水稻(Oryza sativa)Cd胁迫的缓解效应。本研究从广西壮族自治区某矿区Cd污染土壤中分离筛选耐Cd菌株，通过形态学、生理生化特征与16S rDNA基因序列分析法进行鉴定，系统测定菌株的耐Cd特性、环境适应性与植物促生潜能，并采用种子萌发和盆栽试验评价其对水稻Cd污染的缓解促生效果。结果显示，分离获得一株耐Cd优势菌株，最高可耐受1 400 mg/L Cd2+,经鉴定为Cellulosimicrobium cellulans，并命名为C.cellulans C1。菌株C1在pH值为5.0—10.0、NaCl浓度为0—100 g/L条件下均可生长，环境耐受性强，且可通过胞外吸附来钝化Cd2+。此外，菌株C1还具有产1-氨基环丙烷-1-羧酸(ACC)脱氨酶的植物促生活性。Cd胁迫会显著抑制水稻种子萌发与苗期生长，而接种菌株C1后可使水稻种子萌发率由20.00%恢复至52.50%，且苗期中水稻株高、根长、鲜重与干重均较Cd胁迫组显著回升，生长状态基本恢复至对照水平。综上，菌株C1具有高Cd耐受性、吸附钝化能力及产ACC脱氨酶功能，可有效缓解Cd对水稻的毒害作用，在Cd污染农田的微生物修复中具有良好应用潜力。 |
| 关键词: 镉污染 纤维微菌属 耐镉特性 植物促生 水稻 |
| DOI：10.13657/j.cnki.gxkxyxb.20260819.002 |
| 投稿时间：2026-04-05修订日期：2026-04-29 |
| 基金项目:国家自然科学基金项目(32560023)和广西民族大学大学生创新创业训练计划项目(202410608026，202310608017，S202510608309)资助。 |
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| Isolation,Identification of Cadmium-tolerant Strain Cellulosimicrobium cellulans C1 and Its Alleviating Effect on Cadmium Contamination in Rice |
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JI Chunxi, WANG Xuan, LU Junming, SHEN Naikun, ZHANG Hongyan
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| (Guangxi Key Laboratory for Polysaccharide Materials and Modification, School of Marine Sciences and Biotechnology, Guangxi Minzu University, Nanning, Guangxi, 530006, China) |
| Abstract: |
| Isolating highly cadmium (Cd)-tolerant functional strains suitable for the remediation of Cd-polluted farmland helps elucidate their alleviative effects on Cd stress in Oryza sativa.In this study,Cd-tolerant strains were isolated and screened from Cd-contaminated soil collected around a mining area in Guangxi Zhuang Autonomous Region.The strains were identified based on morphological,physiological and biochemical characteristics as well as 16S rDNA gene sequence analysis.We systematically determined their Cd tolerance characteristics,environmental adaptability and plant growth-promoting potential.Furthermore,seed germination tests and pot experiments were conducted to evaluate their alleviating and growth-promoting effects on O.sativa under Cd contamination.A dominant Cd-tolerant strain capable of resisting up to 1 400 mg/L Cd2+ was isolated and identified as Cellulosimicrobium cellulans,which was designated strain C.cellulans C1.The strain C1 could grow under the conditions of pH value of 5.0—10.0 and NaCl concentration of 0—100 g/L,exhibiting strong environmental tolerance.Moreover,it could immobilize Cd2+ via extracellular adsorption.In addition,strain C1 exhibits plant growth-promoting activity by producing 1-aminocyclopropane-1-carboxylate (ACC) deaminase.Cd stress significantly inhibited seed germination and seedling growth of O.sativa.Inoculation with strain C1 increased the O.sativa seed germination rate from 20.00% to 52.50%.Meanwhile,the plant height,root length,fresh weight and dry weight of O.sativa seedlings were markedly elevated compared with the Cd stress group,and the growth status recovered approximately to the control level.In summary,strain C1 has high Cd tolerance,Cd adsorption and immobilization capacity,and the function of producing ACC deaminase.It can effectively alleviate Cd toxicity to rice and has promising application potential for microbial remediation of Cd-contaminated farmland. |
| Key words: cadmium pollution Cellulosimicrobium cadmium tolerance characteristics plant growth promotion Oryza sativa |
