| 引用本文： |
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蒙展彰,黄锦华,原宝龙,张红岩,申乃坤.拮抗金黄色葡萄球菌植物乳植杆菌的筛选鉴定及发酵条件优化[J].广西科学院学报,2026,42(2):143-155. [点击复制]
- MENG Zhanzhang,HUANG Jinhua,YUAN Baolong,ZHANG Hongyan,SHEN Naikun.Screening of Lactic Acid Bacteria with Antagonistic Activity Against Staphylococcus aureus and Optimization of Fermentation Conditions[J].Journal of Guangxi Academy of Sciences,2026,42(2):143-155. [点击复制]
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| 摘要: |
| 为筛选出对金黄色葡萄球菌(Staphylococcus aureus)具有高效拮抗作用的乳酸菌并优化其发酵条件，以S.aureus为指示菌，综合形态学、生理生化及16S rRNA基因序列分析对菌株进行鉴定，并考察其抑菌活性；利用单因素试验和正交试验对菌株抑菌物质发酵工艺进行优化。结果获得一株可高效拮抗S.aureus的乳酸菌S13，经鉴定为植物乳植杆菌(Lactiplantibacillus plantarum)；该菌株的无细胞上清液(Cell-Free Supernatant，CFS)在pH值为 3.0—7.0时对S.aureus具有抑菌活性，可耐高温(40—121 ℃)，且CFS中的抑菌物质可被多种蛋白酶完全消除，初步推断该菌株所产抑菌物质为蛋白类细菌素。优化获得菌株S13的产抑菌物质发酵工艺：初始pH值为6.5、温度为36 ℃、时间为24 h、接种量为3%(V/V)、碳源为20 g/L蔗糖、氮源为19 g/L玉米浆、无机盐为9.25 g/L硫酸锰、吐温80浓度为3 mL/L。在此发酵条件下，菌株S13 CFS的抑制活性较优化前提高14.61%。上述结果表明，筛选得到的L.plantarum S13对S.aureus具有良好的拮抗活性，安全性高，可作为新型食品防腐菌株资源。 |
| 关键词: 金黄色葡萄球菌 植物乳植杆菌 拮抗菌 正交试验 发酵条件优化 |
| DOI：10.13657/j.cnki.gxkxyxb.20260819.003 |
| 投稿时间：2026-03-29修订日期：2026-04-19 |
| 基金项目:国家自然科学基金项目(32560023)和广西民族大学大学生创新创业训练计划项目(202410608026，202310608017，S202510608309)资助。 |
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| Screening of Lactic Acid Bacteria with Antagonistic Activity Against Staphylococcus aureus and Optimization of Fermentation Conditions |
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MENG Zhanzhang, HUANG Jinhua, YUAN Baolong, ZHANG Hongyan, SHEN Naikun
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| (Guangxi Key Laboratory for Polysaccharide Materials and Modification, School of Marine Sciences and Biotechnology, Guangxi Minzu University, Nanning, Guangxi, 530006, China) |
| Abstract: |
| In order to screen lactic acid bacteria exhibiting potent antagonistic effects against Staphylococcus aureus and to optimize their fermentation parameters,S.aureus was employed as the indicator organism.Strain identification was performed based on morphological characteristics,physiological and biochemical properties,and 16S rRNA gene sequencing,followed by assessment of their antibacterial activity.The fermentation conditions for antimicrobial compound production were subsequently optimized via single-factor tests and orthogonal experimental design.As a result,a lactic acid bacterial strain S13 with potent antagonistic activity against S.aureus was obtained and identified as Lactiplantibacillus plantarum.The Cell-Free Supernatant (CFS) of this strain exhibited antibacterial activity against S.aureus within a pH value range of 3.0—7.0 and demonstrated thermostability at temperatures ranging from 40 ℃ to 121 ℃.Moreover,the antibacterial substances present in the CFS could be completely eliminated by various proteases,preliminarily indicating that the antimicrobial compounds produced by this strain are proteinaceous bacteriocins.Through optimization,the optimal fermentation conditions for the production of antibacterial substances by strain S13 were determined as follows:initial pH value 6.5,incubation temperature 36 ℃,fermentation duration 24 h,inoculum concentration 3% (V/V),sucrose as the carbon source at 20 g/L,corn steep liquor as the nitrogen source at 19 g/L,manganese sulfate as the inorganic salt at 9.25 g/L,and Tween 80 at a concentration of 3 mL/L.Under the optimized fermentation conditions,the inhibitory activity of strain S13 CFS was enhanced by 14.61% relative to that prior to optimization.The results indicate that the screened L.plantarum S13 exhibits good antagonistic activity against S.aureus,possesses high safety,and can serve as a novel food preservative strain resource. |
| Key words: Staphylococcus aureus Lactiplantibacillus plantarum antagonistic bacteria orthogonal test optimization of fermentation conditions |
