| 引用本文： |
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李廷章,章双玉,吴书德,廖水意,汤楚乔,李玉玲.青钱柳叶绿体基因组特征分析[J].广西科学院学报,2024,40(4):407-423. [点击复制]
- LI Tingzhang,ZHANG Shuangyu,WU Shude,LIAO Shuiyi,TANG Chuqiao,LI Yuling.Chloroplast Genome and Characteristic Analysis of Cyclocarya paliurus (Batalin) Iljinsk[J].Journal of Guangxi Academy of Sciences,2024,40(4):407-423. [点击复制]
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| 摘要: |
| 青钱柳[Cyclocarya paliurus (Batalin) Iljinsk.]是我国特有的单种属植物，具有重要的药用价值。本研究采用Illumina二代测序技术对青钱柳叶绿体基因组进行测序组装，并对其序列特征、重复序列、密码子偏好性和系统发育进行分析。结果表明：青钱柳叶绿体基因组为典型的环状四分体结构，序列长度为161 291 bp，GC含量为36.00%，共注释得到114个基因，鉴定出136个长重复序列，101个简单重复序列(Simple Sequence Repeat,SSR)，SSR分子标记富含A/T碱基。青钱柳叶绿体基因组50个蛋白编码基因区的密码子整体偏弱；相对同义密码子使用度(Relative Synonymous Codon Usage,RSCU)分析显示，59种密码子偏好使用A和U两种碱基结尾的密码子。基于叶绿体基因组蛋白编码核苷酸序列的系统发育分析表明，化香树族(Platycaryeae Nakai)与胡桃族(Juglandeae Rchb.)呈姐妹群关系，青钱柳属(Cyclocarya Iljinsk.)与胡桃属(Juglans L.)、枫杨属(Pterocarya Kunth)共同形成的分支呈姐妹群关系。本研究可为青钱柳分子标记的开发、物种鉴定、遗传多样性分析及种质资源保护利用提供基础数据。 |
| 关键词: 青钱柳 叶绿体基因组 重复序列 密码子偏好性 系统发育 |
| DOI：10.13657/j.cnki.gxkxyxb.20241226.005 |
| 投稿时间：2023-12-11修订日期：2024-05-14 |
| 基金项目:广东省林木种质资源调查项目(GDZZDC20228703)资助。 |
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| Chloroplast Genome and Characteristic Analysis of Cyclocarya paliurus (Batalin) Iljinsk |
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LI Tingzhang1, ZHANG Shuangyu1, WU Shude2, LIAO Shuiyi2, TANG Chuqiao1, LI Yuling1
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| (1.College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, Guangdong, 510642, China;2.Management Bureau of Guangdong Liannan Bandong Provincial Nature Reserve, Qingyuan, Guangdong, 513300, China) |
| Abstract: |
| Cyclocarya paliurus (Batalin) Iljinsk. is a unique monotypic plant endemic to China,which has important medicinal value. In this study,Illumina second-generation sequencing technology was used to sequence and assemble the chloroplast genome of C.paliurus (Batalin) Iljinsk.,and its sequence characteristics,repetitive sequences,codon preference and phylogeny were analyzed.The results showed that the chloroplast genome of C.paliurus (Batalin) Iljinsk.was a typical circular tetrad structure,with a sequence length of 161 291 bp and a GC content of 36.00%.A total of 114 genes were annotated,136 long repeat sequences and 101 Simple Sequence Repeats (SSR) were identified.SSR molecular markers were rich in A/T bases.The codons of the 50 protein-coding gene regions of the chloroplast genome of C.paliurus (Batalin) Iljinsk.were generally weak.Relative Synonymous Codon Usage (RSCU) analysis showed that 59 codons preferred to utilize A and U as ending codons. Phylogenetic analysis based on chloroplast genome protein-encoded nucleotide sequences showed that Platycaryeae Nakai and Juglandeae Rchb were sister groups,and the branch formed by Cyclocarya Iljinsk.,Juglans L.and Pterocarya Kunth was a sister group. This study can provide basic data for the development of molecular markers,species identification,genetic diversity analysis and germplasm resources protection and utilization of C.paliurus (Batalin) Iljinsk.. |
| Key words: Cyclocarya paliurus (Batalin) Iljinsk. chloroplast genome repeat sequence codon preference phylogeny |
