| 引用本文： |
-
王浩然,解孝满,仝伯强,刘丹,马燕,臧德奎,吴其超.野生玫瑰迁地保存种群遗传多样性保护成效研究[J].广西科学院学报,2024,40(4):424-432. [点击复制]
- WANG Haoran,XIE Xiaoman,TONG Boqiang,LIU Dan,MA Yan,ZANG Dekui,WU Qichao.Study on the Effect of Genetic Diversity Protection of Wild Rose rugosa Ex Situ Conservation Population[J].Journal of Guangxi Academy of Sciences,2024,40(4):424-432. [点击复制]
|
|
| 摘要: |
| 野生玫瑰(Rosa rugosa)具有重要的经济价值、生态价值和育种价值，被列为国家二级重点保护野生植物。了解玫瑰野生种群和迁地保存种群的遗传多样性和遗传结构，可为其种质资源的保护及管理提供指导。本研究基于SSR分子标记对野生玫瑰开展迁地保护成效评估，结果显示：野生种群中以辽宁大连种群的遗传多样性最高，而吉林珲春种群最低。野生种群的总遗传变异(Ht)为0.383 4、种群内遗传变异(Hs)为0.280 3、种群间遗传分化系数(Fst)为0.268 9，遗传变异主要存在于种群内。各种群的观测杂合度(Ho)均小于期望杂合度(He)，杂合子缺失(Fis>0)，群体间亲缘关系较近，存在一定程度的近交。合并后的迁地保存种群的等位基因数(Na)、Shannon's多样性指数(I)、观测杂合度、期望杂合度等均高于野生种群。以上结果说明，迁地保存种群遗传多样性高于野生种群，野生玫瑰的迁地保护对其种群遗传多样性恢复有正向作用，迁地保护成效较好，可为其他野生植物开展迁地保护提供借鉴。 |
| 关键词: 野生玫瑰 迁地保护 成效评估 遗传多样性 遗传结构 SSR |
| DOI：10.13657/j.cnki.gxkxyxb.20241226.006 |
| 投稿时间：2024-06-15修订日期：2024-08-17 |
| 基金项目:山东省重点研发计划(重大科技创新工程)“林木种质资源挖掘与精准鉴定”项目(2021LZGC023)，2021年山东省林业改革发展资金专项“山东省草本植物种质资源普查”项目(鲁财预指〔2021〕1号)，2021年山东省高等学校“青创科技支持计划”“物种保护与生物入侵创新团队”项目(鲁科教涵〔2021〕51号)资助。 |
|
| Study on the Effect of Genetic Diversity Protection of Wild Rose rugosa Ex Situ Conservation Population |
|
WANG Haoran1, XIE Xiaoman2, TONG Boqiang2, LIU Dan2, MA Yan1, ZANG Dekui1, WU Qichao1
|
| (1.Key Laboratory of Afforestation in the Lower Reaches of the Yellow River, National Forestry and Grassland Administration, Forestry College, Shandong Agricultural University, Tai'an, Shandong, 271018, China;2.Center for Forest and Grass Genetic Resources of Shandong Province, Jinan, Shandong, 250014, China) |
| Abstract: |
| Wild rose (Rosa rugosa) has important economic value,ecological value and breeding value,and it is listed as a national second-class key protected wild plant.Understanding the genetic diversity and genetic structure of wild populations and ex situ conservation populations of R.rugosa can provide guidance for the protection and management of its germplasm resources.In this study,the effectiveness of ex situ conservation of wild R.rugosa was evaluated based on SSR molecular markers.The results showed that the genetic diversity of the wild rose population in Dalian,Liaoning Province was the highest,while that in Hunchun,Jilin Province was the lowest.The total genetic variation (Ht) of the wild population was 0.383 4,the genetic variation (Hs) within the population was 0.280 3,and the genetic differentiation coefficient (Fst) between populations was 0.268 9.The genetic variation mainly existed within the population.The observed heterozygosity (Ho) of each population was less than the expected heterozygosity (He),and the heterozygotes were missing (Fis >0).The genetic relationship between populations was close,and there was a certain degree of inbreeding.The number of alleles (Na),Shannon's diversity index (I),observed heterozygosity (Ho) and expected heterozygosity (He) of the combined ex situ conservation population were higher than those of the wild population.The above results show that the genetic diversity of ex situ conservation population is higher than that of wild population.The ex situ conservation of wild rose has a positive effect on the recovery of genetic diversity of its population,and the effect of ex situ conservation is better,which can provide a reference for other wild plants to carry out ex situ conservation. |
| Key words: wild rose relocation protection effectiveness evaluation genetic diversity genetic structure SSR |
