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黄霖,潘绘竹,李杰,谢芳,韦维,邢政,赖俊翔.海洋卡盾藻对链状裸甲藻的化感作用研究[J].广西科学院学报,2024,40(4):400-406. [点击复制]
- HUANG Lin,PAN Huizhu,LI Jie,XIE Fang,WEI Wei,XING Zheng,LAI Junxiang.Allelopathic Effect of Chattonella marina on Gymnodinium catenatum[J].Journal of Guangxi Academy of Sciences,2024,40(4):400-406. [点击复制]
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| 海洋卡盾藻对链状裸甲藻的化感作用研究 |
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黄霖1,2, 潘绘竹2,3, 李杰2,3, 谢芳1,2, 韦维1,2, 邢政1,2, 赖俊翔2,3
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| (1.广西大学资源环境与材料学院, 广西南宁 530006;2.广西科学院, 广西海洋科学院(广西红树林研究中心), 广西近海海洋与环境科学重点实验室, 广西南宁 530006;3.北部湾海洋产业研究院, 广西防城港 538000) |
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| 摘要: |
| 链状裸甲藻(Gymnodinium catenatum)是我国东南沿海常见的有毒赤潮原因种之一，其产生的麻痹性贝类毒素(PSP)给渔业带来了巨大的经济损失，且该问题在全球范围内日益严重。有研究表明，海洋卡盾藻(Chattonella marina)对链状裸甲藻有明显的化感抑制作用，但是对其化感作用机制鲜有研究。本研究结合超氧阴离子自由基(O-2·)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、丙二醛(MDA)、Caspase-3等指标，对海洋卡盾藻次级代谢产物中主要化感物质的抑藻效果进行分析，发现海洋卡盾藻化感物质的氧化胁迫是诱导链状裸甲藻细胞凋亡的主要原因。相较于海洋卡盾藻中其他次级代谢产物，棕榈油酸是抑藻活性最好的化感物质，棕榈油酸能够显著地抑制链状裸甲藻细胞的生长，抑制效果随着棕榈油酸浓度的增大而增强，并具有“低促高抑”的毒物兴奋效应。此外，棕榈油酸能够对链状裸甲藻的抗氧化系统造成影响，导致链状裸甲藻细胞内O-2·过度积累而引起膜脂过氧化,Caspase-3含量增加，进一步诱导链状裸甲藻细胞凋亡。该研究为理解海洋环境中微藻间化感作用的生态影响和作用机制提供了新的视角，对预测全球变化背景下海洋初级生产力动态、赤潮生物暴发机制及微型生态系统稳定性具有一定参考意义。 |
| 关键词: 链状裸甲藻 海洋卡盾藻 化感作用 棕榈油酸 氧化胁迫 |
| DOI：10.13657/j.cnki.gxkxyxb.20241226.004 |
| 投稿时间：2024-03-20修订日期：2024-06-26 |
| 基金项目:国家重点研发计划重点专项(2022YFD2401200)和中央引导地方科技发展资金项目(桂科ZY21195027)资助。 |
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| Allelopathic Effect of Chattonella marina on Gymnodinium catenatum |
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HUANG Lin1,2, PAN Huizhu2,3, LI Jie2,3, XIE Fang1,2, WEI Wei1,2, XING Zheng1,2, LAI Junxiang2,3
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| (1.School of Resources, Environment and Materials, Guangxi University, Nanning, Guangxi, 530006, China;2.Guangxi Key Laboratory of Marine and Coastal Environmental Sciences, Guangxi Academy of Marine Sciences (Guangxi Mangrove Research Center), Guangxi Academy of Sciences, Nanning, Guangxi 530006, China;3.Beibu Gulf Marine Research Institute, Fangchenggang, Guangxi, 538000, China) |
| Abstract: |
| Gymnodinium catenatum is one of the common toxic red tide causative species in the coastal waters of Southeast China.The Paralytic Shellfish Poisoning (PSP) produced by G.catenatum has brought huge economic losses to fisheries,and the problem is becoming more and more serious worldwide.Studies have shown that Chattonella marina has a significant allelopathic inhibitory effect on G.catenatum, but there is little research on its allelopathic mechanism.This study combined indicators such as superoxide anion radicals (O-2·),superoxide dismutase (SOD),catalase (CAT),malondialdehyde (MDA),and Caspase-3 to analyze the algicidal effect of the main allelochemicals in the secondary metabolites of C.marina, and it was found that oxidative stress caused by allelochemicals of C.marina was the main reason for inducing apoptosis in G.catenatum cells.Compared with other secondary metabolites in C.marina, palmitoleic acid is the best allelochemical with algae inhibition activity.Palmitoleic acid can significantly inhibit the growth of G.catenatum cells,and the inhibition effect increases with the increase of palmitoleic acid concentration,and has a toxic excitatory effect of “low promotion and high inhibition”.In addition,palmitoleic acid can affect the antioxidant system of G.catenatum, leading to excessive accumulation of O-2· in the cells and causing lipid peroxidation of the membrane,and an increase in Caspase-3 content,further inducing apoptosis in G.catenatum cells.This study provides a new perspective for understanding the ecological impact and mechanism of allelopathy among microalgae in the marine environment.It has certain reference significance for predicting the dynamics of marine primary productivity,the mechanism of red tide organism outbreaks,and the stability of micro-ecosystems under the background of global change. |
| Key words: Gymnodinium catenatum Chattonella marina allelopathic palmitoleic acid oxidative damage |
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