| 摘要: |
| 本研究旨在探讨围填海对白骨壤（Avicennia marina）叶片叶绿素含量、呼吸与能量代谢的影响。实验以围填海不同区域白骨壤的叶片和指状呼吸根为材料，研究围填海对白骨壤叶片叶绿素含量、NAD+/NADH比值、NADP+/NADPH比值、ATP含量和指状呼吸根活力、细胞色素C氧化酶和乙醇脱氢酶活性的影响。实验结果表明，围填海使白骨壤叶片叶绿素含量平均下降53%，NAD+/NADH比值下降61%，NADP+/NADPH比值下降62%，ATP含量下降95%，指状呼吸根活力下降62%，细胞色素C氧化酶活性升高108%，乙醇脱氢酶活性升高20%。高岭土覆盖在白骨壤叶片表面，使其可利用的光能减少，导致其叶绿素含量降低；白骨壤叶片和指状呼吸根气孔被高岭土堵塞，呼吸及能量代谢受阻。因此，光照不足及低氧胁迫导致白骨壤死亡。 |
| 关键词: 白骨壤 围填海 叶绿素 细胞色素C氧化酶 乙醇脱氢酶 |
| DOI：10.13657/j.cnki.gxkxyxb.20210927.002 |
| 投稿时间：2021-06-02 |
| 基金项目:合浦县林业局项目（GXZC2020-J3-HXHP-001），广西自然科学基金项目（2020GXNSFAA297067，2018GXNSFBA281062）和广西红树林保护与利用重点实验室基金项目（GKLMC-20A04）资助。 |
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| Effects of Reclamation on Respiration and Energy Metabolism of Avicennia marina |
|
CHEN Siting
|
| (Guangxi Key Lab of Mangrove Conservation and Utilization, Guangxi Mangrove Research Center, Guangxi Academy of Sciences, Beihai, Guangxi, 536000, China) |
| Abstract: |
| This study aimed to investigate the effects of reclamation on chlorophyll content, respiration and energy metabolism in leaves of Avicennia marina. In the experiment, the leaves and finger-like pneumetophores of A. marina from different areas of reclamation were used as materials. The effects of reclamation on the chlorophyll content, NAD+/NADH ratio、NADP+/NADPH ratio, ATP content, finger-like pneumetophores viability, the activities of cytochrome C oxidase and alcohol dehydrogenase of A. marina leaves were studied. The experimental results showed that the reclamation decreased the average chlorophyll content, NAD+/NADH ratio, NADP+/NADPH ratio, ATP content and finger-like pneumetophores viability by 53%, 61%, 62%, 95%, 62%, respectively. And activities of cytochrome C oxidase and alcohol dehydrogenase increased by 108% and 20%, respectively. The results showed that kaolinite was covered on the leaf surface of A. marina, which reduced the available light energy and led to the decrease of chlorophyll content. The stomata in leaves and pneumetophores of A. marina were blocked by kaolinite, and the respiration and energy metabolism were blocked. Therefore, insufficient light and hypoxia stress lead to the death of A. marina. |
| Key words: Avicennia marina reclamation chlorophyll cytochrome C oxidase alcohol dehydrogenase |
