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刘明珠,李梦梦,黄帅帅,余庆,徐凤巧,李鹏飞.威灵仙水提物对卵形鲳鲹源溶藻弧菌的抑制作用[J].广西科学院学报,2021,37(2):94-100. [点击复制]
- LIU Mingzhu,LI Mengmeng,HUANG Shuaishuai,YU Qing,XU Fengqiao,LI Pengfei.Inhibitory Effect of Clematis chinensis Osbeck Against Vibrio alginolyticus from Trachinotus ovatus[J].Journal of Guangxi Academy of Sciences,2021,37(2):94-100. [点击复制]
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| 威灵仙水提物对卵形鲳鲹源溶藻弧菌的抑制作用 |
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刘明珠1, 李梦梦2, 黄帅帅3, 余庆1, 徐凤巧1,4, 李鹏飞1,2
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| (1.广西壮族自治区渔业重大疫病防控与高效健康养殖产业技术工程研究中心, 广西南宁 530007;2.河南师范大学生命科学学院, 河南新乡 453007;3.北部湾大学海洋学院, 广西北部湾海洋生物多样性养护重点实验室, 广西钦州 535011;4.广西科学院, 广西海洋天然产物与组合生物合成化学重点实验室, 广西南宁 530007) |
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| 摘要: |
| 近年来水产养殖行业不断扩大，但随之而来的鱼病对养殖行业造成了巨大损失。本研究以卵形鲳鲹（前期发病）上分离出的溶藻弧菌为实验对象，批量筛选多种中草药，选择抑菌效果最好的一种或几种中草药，并探究不同浓度中草药水提物的抑菌效果，确定其杀菌浓度（MBC）及最小抑菌浓度（MIC），再通过生化指标胞内乳酸脱氢酶（Lactate dehydrogenase，LDH）的含量来确定其杀菌机制。对大量中草药水提物的筛选结果表明，威灵仙水提物对溶藻弧菌具有较好的抑制作用；通过二倍稀释法将威灵仙水提物进行逐级稀释后，发现其对溶藻弧菌的致死浓度为500 mg/mL，最小抑菌浓度为250 mg/mL；孵育2 h后，实验组胞内LDH的含量明显比对照组含量低，并且随着时间的推移，对照组含量先升高后降低，而实验组含量逐渐减少，实验组与对照组胞内LDH含量出现显著性的差异。威灵仙水提物致死溶藻弧菌有可能是通过破坏细胞膜，使细胞内容物释放从而引起菌体裂解。 |
| 关键词: 威灵仙 卵形鲳鲹 溶藻弧菌 抗菌活性 LDH含量 |
| DOI：10.13657/j.cnki.gxkxyxb.20210809.001 |
| 投稿时间：2021-05-02 |
| 基金项目:国家自然科学基金项目（U20A20102，41966004），广西重点研发计划项目（桂科AB18221111），广西自然科学基金项目（2020GXNSFBA297161，2019GXNSFAA245054，AD19245022）和广西科学院基本科研业务费项目（2019YJJ1005，2018YJJ903）资助。 |
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| Inhibitory Effect of Clematis chinensis Osbeck Against Vibrio alginolyticus from Trachinotus ovatus |
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LIU Mingzhu1, LI Mengmeng2, HUANG Shuaishuai3, YU Qing1, XU Fengqiao1,4, LI Pengfei1,2
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| (1.Guangxi Engineering Research Center for Fishery Major Diseases Control and Efficient Healthy Breeding Industrial Technology, Nanning, Guangxi, 530007, China;2.College of Life Science, Henan Normal University, Xinxiang, Henan, 453007, China;3.Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, College of Marine Sciences, Beibu Gulf University, Qinzhou, Guangxi, 535011, China;4.Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthetic Chemistry, Guangxi Academy of Sciences, Nanning, Guangxi, 530007, China) |
| Abstract: |
| In recent years, with the expansion of aquaculture, the ensuing fish disease has caused great losses in aquaculture industry. In this study, Vibrio alginolyticus separated from sick Trepanotus ovatus was used as the experimental object, a large number of Chinese medicines in batches were screened, and one or several Chinese medicines with the best antibacterial effect were selected. It was explored that the antibacterial effect of water extracts with different concentrations. It was determined that the bactericidal concentration (MBC) and minimum inhibitory concentration (MIC), and the bactericidal mechanism were determined by the content of intracellular lactate dehydrogenase (LDH). By the results of screening a large number of water extracts of traditional Chinese medicine, it was confirmed that Clematis chinensis Osbeck had a good inhibitory effect to V. alginolyticus. The screening results of a large number of water extracts of traditional Chinese medicine showed that C. chinensis Osbeck had a good inhibitory effect on V. alginolyticus. After diluting Clematis chinensis Osbeck step by step by double dilution method, it was found that its lethal concentration to V. alginolyticus was 500 mg/mL, and the minimum inhibitory concentration was 250 mg/mL. After 2 h of incubation, the intracellular LDH content in the experimental group was significantly lower than that in the control group, and with the passage of time, the intracellular LDH content in the control group first increased and then decreased, while the content of the experimental group gradually decreased. The intracellular LDH content of the experimental group and the control group showed a significant difference. The death mechanism of C. chinensis Osbeck induced V. alginolyticus may be caused by destruction of cell membrane to release cell contents and lead to cell lysis. |
| Key words: Clematis chinensis Osbeck Trachinotus ovatus Vibrio alginolyticus antimicrobial activity LDH content |
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