| 引用本文： |
-
黄帅帅,余庆,刘明珠,李梦梦,徐凤巧,李鹏飞.秦皮水提物和秦皮乙素抗石斑鱼虹彩病毒的研究[J].广西科学院学报,2021,37(2):85-93. [点击复制]
- HUANG Shuaishuai,YU Qing,LIU Mingzhu,LI Mengmeng,XU Fengqiao,LI Pengfei.Study on the Antiviral Abilities of Cortex Fraxini Water Extracts and Esculetin Against Grouper Iridovirus Infection[J].Journal of Guangxi Academy of Sciences,2021,37(2):85-93. [点击复制]
|
|
| |
|
|
| 本文已被：浏览 430次 下载 320次 |
码上扫一扫！ |
| 秦皮水提物和秦皮乙素抗石斑鱼虹彩病毒的研究 |
|
黄帅帅1,2, 余庆2, 刘明珠2, 李梦梦3, 徐凤巧2, 李鹏飞1,2
|
|
|
| (1.北部湾大学海洋学院, 广西北部湾海洋生物多样性养护重点实验室, 广西钦州 535011;2.广西科学院, 广西壮族自治区渔业重大疫病防控与高效健康养殖产业技术工程研究中心, 广西海洋天然产物与组合生物合成化学重点实验室, 广西南宁 530007;3.河南师范大学生命科学学院, 河南新乡 453007) |
|
| 摘要: |
| 为开发绿色、安全、高效的抗石斑鱼虹彩病毒（Singapore Groupe Riridovirus，SGIV）渔用药物，本研究先采用光学显微镜观察、CCK-8细胞活力测定实验确定秦皮水提物（Cortex Fraxini water extracts，CFE）及其主要活性成分秦皮乙素（Esculetin，EC）的细胞安全浓度，并在确定药物安全工作浓度基础上，分别采用光学显微镜观察、实时荧光定量PCR（Realtime Quantitative，RT-qPCR）、基于核酸适配体LYGV1的荧光分子探针技术（LYGV1-AFMP）等方法探究CFE和EC抗石斑鱼虹彩病毒的效果。结果表明，当CFE浓度低于1 mg/mL、EC浓度低于10 μg/mL时，光学显微镜观察结果显示细胞形态正常，无明显病变效应。光学显微镜观测结果与CCK-8检测细胞存活率结果一致，即秦皮水提物安全工作浓度为1 mg/mL（ρCFE ≤ 1 g/L），秦皮乙素安全工作浓度为 ≤ 10 μg/mL（ρEC ≤ 0.01 g/L）。光学显微镜观察、RT-qPCR、LYGV1-AFMP这3种检测技术均证实秦皮水提物及秦皮乙素具有明显的抗石斑鱼虹彩病毒效果，且在安全工作浓度下，抗病毒效果与药物剂量呈正相关关系。秦皮水提物及秦皮乙素能够有效抑制石斑鱼虹彩病毒的感染，具有开发成高效抗病毒渔用药物的潜力。 |
| 关键词: 秦皮 秦皮乙素 石斑鱼虹彩病毒 渔用药物 抗病毒 |
| DOI：10.13657/j.cnki.gxkxyxb.20210806.001 |
| 投稿时间：2021-05-01 |
| 基金项目:国家自然科学基金项目（41966004，U20A20102），广西重点研发计划项目（桂科AB18221111），广西自然科学基金项目（2020GXNSFBA297161，2019GXNSFAA245054，AD19245022）和广西科学院基本科研业务费项目（2019YJJ1005，2018YJJ903）资助。 |
|
| Study on the Antiviral Abilities of Cortex Fraxini Water Extracts and Esculetin Against Grouper Iridovirus Infection |
|
HUANG Shuaishuai1,2, YU Qing2, LIU Mingzhu2, LI Mengmeng3, XU Fengqiao2, LI Pengfei1,2
|
| (1.Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, College of Marine Sciences, Beibu Gulf University, Qinzhou, Guangxi, 535011, China;2.Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry, Guangxi Engineering Research Center for Fishery Major Diseases Control and Efficient Healthy Breeding Industrial Technology, Guangxi Academy of Sciences, Nanning, Guangxi, 530007, China;3.College of Life Science, Henan Normal University, Xinxiang, Henan, 453007, China) |
| Abstract: |
| In order to develop green, safe and efficient drugs against grouper iridovirus (SGIV), this article first uses optical microscope observation and CCK-8 cell viability test to determine the safe cell concentration of Cortex Fraxini water extracts (CFE) and its main active ingredient Esculetin (EC). And on the basis of determining the safe working concentration of the drug, the methods of optical microscope observation, real-time fluorescent quantitative PCR (Real-time quantitative PCR, RT-qPCR), and fluorescent molecular probe technology (LYGV1-AFMP) based on nucleic acid aptamer LYGV1 (LYGV1-AFMP) were used to explore CFE and the effect of EC against grouper iridovirus. The results show that when the CFE concentration was lower than 1 mg/mL and the EC concentration is lower than 10 μg/mL, the light microscope observation results show that the cell morphology was normal and there is no obvious pathological effect. The results of optical microscope observation are consistent with the results of CCK-8 detection of cell viability, that was, the safe working concentration of CFE is 1 mg/mL (ρCFE ≤ 1 g/L), and the safe working concentration of Esculetin is ≤ 10 μg/mL (ρEC ≤ 0.01 g/L). The light microscope observation, RT-qPCR and LYGV1-AFMP all confirm that CFE and Esculetin, have great anti-SGIV effects, and the anti-SGIV effects were positively correlated with the drug amount at safe working concentration. Cortex Fraxini water extracts and its main active ingredient Esculetin could effectively inhibit SGIV infection, and have the potential to develop highly effective antiviral drugs for aquaculture. |
| Key words: Cortex Fraxini Esculetin singapore grouper iridovirus fishery drugs hamper the virus |
|
|
|
|
|
