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黄连水提物对卵形鲳鲹源溶藻弧菌的抑菌作用
刘明珠1, 肖贺贺2, 余庆3, 覃仙玲3, 黎思巧3, 陆兰天3, 吴思婷1, 王一兵4, Dedi Fazriansyah Putra5, 王太霞2, 李鹏飞3
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(1.广西科学院, 广西海洋研究所, 广西海洋生物技术重点实验室, 广西北海 536000;2.河南师范大学生命科学学院, 河南新乡 453007;3.广西近海海洋环境科学重点实验室, 广西南宁 530007;4.广西民族大学海洋与生物技术学院, 广西南宁 530006;5.印度尼西亚亚齐大学, 班达亚齐 999006)
摘要:
卵形鲳鲹(Trachinotus ovatus)是一种生长快速且肉质鲜美的经济型海水养殖鱼类,但是近些年为满足人们的日常需求,在不断扩大养殖的同时病菌也逐渐泛滥。溶藻弧菌(Vibrio alginolyticus)是华南沿海地区海水养殖鱼类细菌性鱼病的主要致病菌之一,给水产养殖业带来巨大损失。本研究对黄连(Coptis chinensis Franch)的抗菌作用进行系统研究,目的是为由溶藻弧菌引起的爆发性细菌性鱼病的高效防治提供科学的用药依据。本研究采用二倍稀释法测定黄连水提物对卵形鲳鲹源溶藻弧菌的最低抑菌浓度(MIC)、最低杀菌浓度(MBC)和半数致死量(LD50),并使用BCA蛋白浓度测定试剂盒测定细菌超声破碎上清液中可溶性蛋白含量。结果表明,黄连水提物对溶藻弧菌具有明显的抑制作用,其对溶藻弧菌的MIC、MBC和LD50的值分别为7.800 mg/mL、31.250 mg/mL和15.625 mg/mL。胞内可溶性蛋白含量变化的结果提示:黄连发挥抑菌作用的机制可能是通过损伤细菌的细胞壁导致细菌内容物的释放并引起菌体裂解死亡。黄连具有发展成为一种高效抗水产病害中草药制剂的潜力。
关键词:  黄连  卵形鲳鲹  溶藻弧菌  细胞毒性  抗菌活性
DOI:10.13657/j.cnki.gxkxyxb.20190515.003
基金项目:广西重点研发计划(2018AB52003),广西自然科学基金项目(2017GXNSFBA198176),中央引导地方科技发展专项(2018ZYZX2036),广西科学院基本科研业务费项目(2018YJJ903,2017YJJ23002)和广西南海珊瑚礁研究重点实验室开放课题(GXLSCRSCS2018003)资助。
Antimicrobial Effect of Water Extracts of Coptis chinensis Franch Against Vibrio alginolyticus from Trachinotus ovatus
LIU Mingzhu1, XIAO Hehe2, YU Qing3, QIN Xianling3, LI Siqiao3, LU Lantian3, WU Siting1, WANG Yibing4, Dedi Fazriansyah Putra5, WANG Taixia2, LI Pengfei3
(1.Guangxi Key Lab for Marine Biotechnology, Guangxi Institute of Oceanography, Guangxi Academy of Sciences, Beihai, Guangxi, 536000, China;2.College of Life Science, Henan Normal University, Xinxiang, Henan, 453007, China;3.Guangxi Key Laboratory of Marine Environmental Science, Nanning, Guangxi, 530007, China;4.School of Marine Sciences and Biotechnology, Guangxi University for Nationalities, Nanning, Guangxi, 530006, China;5.Department of Aquaculture, Syiah Kuala University, Banda Aceh, 999006, Indonesia)
Abstract:
Trachonotus ovatus is an economical marine fish that grows fast and has a delicious taste. However, in recent years, in order to meet people's daily needs, the germs are gradually spreading under the continuous expansion of breeding. Vibrio alginolyticus is one of the main pathogens of bacterial fish disease in marine fish in coastal areas of South China, causing huge losses to the aquaculture industry. In this paper, the antimicrobial effects of Coptis chinensis Franch was systematically studied to provide a scientific basis for the effective prevention and treatment of explosive bacterial fish disease caused by Vibrio alginolyticus.In this study, the minimum inhibitory concentration (MIC), the minimum bactericidal concentration (MBC) and the median lethal dose (LD50) of the aqueous extract of C.chinensis against V.alginolyticus from T.ovatus were determined by two-fold dilution method. After sonic disruption, the soluble protein contents in V.alginolyticus were determined by BCA protein concentration assay kit. The experiment showed that the water extracts of C.chinensis had obvious inhibitory effects on V.alginolyticus,and the MIC, MBC and LD50 values of V.alginolyticus were 7.800 mg/mL, 31.250 mg/mL and 15.625 mg/mL, respectively. The changes of soluble protein contents in supernatants suggest that C.chinensis can play the inhibitory role by damaging the bacterial cell wall and causing the release of bacterial contents, which results in the lysis and death of bacteria.C.chinensis has the potential to develop a highly effective Chinese herbal preparation against aquatic diseases.
Key words:  Coptis chinensis  Trachinotus ovatus  Vibrio alginolyticus  cytotoxic  antimicrobial activity

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