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广西莪术水提物对卵形鲳鲹细胞免疫力的影响
刘明珠1, 肖贺贺2, 余庆3, 吴思婷1, 覃仙玲3, 陈宪云3, 黎思巧3, 王一兵4, Dedi Fazriansyah Putra5, 王太霞2, 李鹏飞3
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(1.广西科学院, 广西海洋研究所, 广西海洋生物技术重点实验室, 广西北海 536000;2.河南师范大学生命科学学院, 河南新乡 453007;3.广西近海海洋环境科学重点实验室, 广西南宁 530007;4.广西民族大学海洋与生物技术学院, 广西南宁 530006;5.印度尼西亚亚齐大学, 班达亚齐 999006)
摘要:
卵形鲳鲹(Trachinotus ovatus)是我国重要的海水养殖鱼类,经济价值极高,但近年来各种鱼病频繁爆发造成了巨大损失。为探究广西莪术(Curcuma kwangsiensis S.G.Lee et C.F.Liang)水提物对卵形鲳鲹免疫力的影响,本文在细胞水平上,利用卵形鲳鲹肾脏组织细胞系研究广西莪术水提物的细胞毒性,以确定其细胞安全浓度。然后利用卵形鲳鲹细胞系研究广西莪术水提物对总一氧化氮合酶(T-NOS)、酸性磷酸酶(ACP)、超氧化物歧化酶(SOD)和溶菌酶等卵形鲳鲹细胞免疫相关酶的影响。结果表明,广西莪术水提物的细胞安全浓度为≤ 5 mg/mL,广西莪术水提物对卵形鲳鲹细胞的免疫相关酶活力(总一氧化氮合酶、酸性磷酸酶、超氧化物歧化酶、溶菌酶)有明显促进作用。广西莪术有潜力发展成为一种高效的水产免疫增强剂,并且基于传统壮药积极开展绿色无药物残留的新型抗水产病害中药制剂的研究,对于促进水产养殖业持续健康发展,确保水产品质量和食品安全意义重大。
关键词:  卵形鲳鲹  广西莪术  细胞毒性  免疫酶活力
DOI:10.13657/j.cnki.gxkxyxb.20190515.004
基金项目:广西重点研发计划(2018AB52003),广西自然科学基金项目(2017GXNSFBA198176),中央引导地方科技发展专项(2018ZYZX2036)和广西科学院基本科研业务费项目(2018YJJ903,2017YJJ23002)资助。
Effects of Curcuma kwangsiensis S.G.Lee et C.F.Liang on Immune Activities of Trachinotus ovatus Cells
LIU Mingzhu1, XIAO Hehe2, YU Qing3, WU Siting1, QIN Xianling3, CHEN Xianyun3, LI Siqiao3, WANG Yibing4, Dedi Fazriansyah Putra5, WANG Taixia2, LI Pengfei3
(1.Guangxi Key Lab for Marine Biotechnology, Guangxi Institute of Oceanography, Guangxi Academy of Sciences, Beihai, Guangxi, 536000, China;2.College of Life Science, Henan Normal University, Xinxiang, Henan, 453007, China;3.Guangxi Key Laboratory of Marine Environmental Science, Nanning, Guangxi, 530007, China;4.School of Marine Sciences and Biotechnology, Guangxi University for Nationalities, Nanning, Guangxi, 530006, China;5.Department of Aquaculture, Syiah Kuala University, Banda Aceh, 999006, Indonesia)
Abstract:
Trachinotus ovatus is an important marine fish with high economic value in China. However, the frequent outbreak of various fish diseases has caused great losses in recent years. In order to investigate the effects of Curcuma kwangsiensis S.G.Lee et C.F.Liang on the immunity of T.ovatus,at the cellular level, the cytotoxicity of water extracts of Curcuma kwangsiensis S.G.Lee et C.F.Liang in Guangxi was studied using the ovate-shaped kidney tissue cell line to determine the safe concentration of cells. Then, the effect of water extracts of Curcuma kwangsiensis S.G.Lee et C.F.Liang on the immune-related enzymes of ovate-shaped cells which included total nitrogen monoxide synthase (T-NOS), acid phosphatase (ACP), superoxide dismutase (SOD) and lysozyme was studied by using the ovate cell line. The safe concentration of Curcuma kwangsiensis S.G.Lee et C.F.Liang water extracts was ≤ 5 mg/mL.Curcuma kwangsiensis S.G.Lee et C.F.Liang could obviously promote the activity of immune-related enzymes (T-NOS, ACP, SOD and lysozyme) in TOK cells.Curcuma kwangsiensis S.G.Lee et C.F.Liang has potential to develop into a highly effective aquaculture immune enhancer. Based on the traditional Chinese Zhuang medicine, it is of great significance to carry out the research on new Chinese herbal preparations against aquatic diseases, which could promote the sustainable and healthy development of aquaculture and ensure the quality of aquatic products and food safety.
Key words:  Trachinotus ovatus  Curcuma kwangsiensis S.G.Lee et C.F.Liang  cytotoxicity  immune-related enzymes

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