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  • 赵湘培,郭力城,滕红丽.薄层色谱法评价不同产地土茯苓的品质[J].广西科学院学报,2015,31(4):308-310.    [点击复制]
  • ZHAO Xiang-pei,GUO Li-cheng,TENG Hong-li.Identification of Smilax glabra Roxb.from Different Origin by TLC[J].Journal of Guangxi Academy of Sciences,2015,31(4):308-310.   [点击复制]
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薄层色谱法评价不同产地土茯苓的品质
赵湘培, 郭力城, 滕红丽
0
(广西壮族自治区民族医药研究院/广西壮医医院, 广西南宁 530001)
摘要:
[目的]建立评价土茯苓(Smilax glabra Roxb.)品质的薄层色谱分析方法。[方法]以有效成分落新妇苷为指标,以氯仿-甲醇-甲酸(4:1:0.05)展开系统,建立土茯苓的专属性薄层色谱,并评价10个不同产地土茯苓的品质。[结果]不同产地土茯苓药材在与对照色谱相应位置上,显示出相同颜色的荧光斑点,且斑点清晰,比移值(Rf)为0.41。[结论]薄层色谱法能够有效对土茯苓进行鉴别,且该方法简便、准确,具有较好的适应性。
关键词:  土茯苓  落新妇苷  薄层色谱
DOI:
投稿时间:2015-04-10
基金项目:国家科技支撑计划项目(2012BAI27B06-1,2013BAI11B06-6),广西重大专项计划项目(桂科重14124002-14)和南宁市科技攻关与新产品试制项目(20133158,20133163)资助。
Identification of Smilax glabra Roxb.from Different Origin by TLC
ZHAO Xiang-pei, GUO Li-cheng, TENG Hong-li
(Guangxi Institute of Minority Medicine/Traditional Zhuang Hospital of Guangxi, Nanning, Guangxi, 530001, China)
Abstract:
[Objective] To establish a TLC method for the identification of Smilax glabra Roxb..[Methods] Astilbin used as indicators,chloroform-methanol-formic acid (4:1:0.05) used as developing agent.[Results] The same color spots were shown in the corresponding position of the Smilax glabra Roxb.from different origin,and the spots were clear. The Rf index was 0.41.[Conclusion] The TLC method is effective for the identification of the Smilax glabra Roxb.,which is simple and reliable.
Key words:  Smilax glabra Roxb.  astilbin  TLC

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