引用本文: |
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陈秋虹,徐慧,蒋艳芳.萝芙木药材中总生物碱的含量测定方法研究[J].广西科学院学报,2010,26(3):327-328. [点击复制]
- CHEN Qiu-hong,XU Hui,JIANG Yan-fang.Research on Determination of Total Alkaloid in Radix Rauvolfiae Verticillatae[J].Journal of Guangxi Academy of Sciences,2010,26(3):327-328. [点击复制]
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摘要: |
用氯仿提取总生物碱,通过酸性染料比色法测定萝芙木药材中总生物碱含量。结果萝芙木生物碱在8.0~24.0μg具有良好的线性关系(r=0.9914),平均回收率为100.1%,RSD为0.65%。该方法简单,准确,可用于萝芙木药材的质量控制。 |
关键词: 酸性染料比色法 总生物碱 萝芙木 含量测定 |
DOI: |
投稿时间:2010-06-20 |
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Research on Determination of Total Alkaloid in Radix Rauvolfiae Verticillatae |
CHEN Qiu-hong, XU Hui, JIANG Yan-fang
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(Guangxi Research Center of Analysis and Testing, Nanning, Guangxi, 530022, China) |
Abstract: |
The total alkaloid in Radix Rauvolfiae Verticillatae was extracted by chloroform.The contents of the total alkaloid was calculated through the acidic dye color metric method.The result showed that:the calibration curve of total alkaloid in Radix Rauvolfiae Verticillatae was linear in the range from 8.0μg to 24.0μg and correlation cofficient was 0.9914.The average recovery was 100.1% and the RSD was 0.65%.This method was proved to be simple, accurate and can be used for the quality control of Radix Rauvolfiae Verticillatae. |
Key words: acidic dye color metric method total alkaloid determination Radix Rauvolfiae Verticillatae |