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  • 陈秋虹,徐慧,蒋艳芳.萝芙木药材中总生物碱的含量测定方法研究[J].广西科学院学报,2010,26(3):327-328.    [点击复制]
  • CHEN Qiu-hong,XU Hui,JIANG Yan-fang.Research on Determination of Total Alkaloid in Radix Rauvolfiae Verticillatae[J].Journal of Guangxi Academy of Sciences,2010,26(3):327-328.   [点击复制]
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萝芙木药材中总生物碱的含量测定方法研究
陈秋虹, 徐慧, 蒋艳芳
0
(广西分析测试研究中心 530022)
摘要:
用氯仿提取总生物碱,通过酸性染料比色法测定萝芙木药材中总生物碱含量。结果萝芙木生物碱在8.0~24.0μg具有良好的线性关系(r=0.9914),平均回收率为100.1%,RSD为0.65%。该方法简单,准确,可用于萝芙木药材的质量控制。
关键词:  酸性染料比色法  总生物碱  萝芙木  含量测定
DOI:
投稿时间:2010-06-20
基金项目:
Research on Determination of Total Alkaloid in Radix Rauvolfiae Verticillatae
CHEN Qiu-hong, XU Hui, JIANG Yan-fang
(Guangxi Research Center of Analysis and Testing, Nanning, Guangxi, 530022, China)
Abstract:
The total alkaloid in Radix Rauvolfiae Verticillatae was extracted by chloroform.The contents of the total alkaloid was calculated through the acidic dye color metric method.The result showed that:the calibration curve of total alkaloid in Radix Rauvolfiae Verticillatae was linear in the range from 8.0μg to 24.0μg and correlation cofficient was 0.9914.The average recovery was 100.1% and the RSD was 0.65%.This method was proved to be simple, accurate and can be used for the quality control of Radix Rauvolfiae Verticillatae.
Key words:  acidic dye color metric method  total alkaloid  determination  Radix Rauvolfiae Verticillatae

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