| 引用本文： |
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陈勇,韦韬,魏后超,卢森华,曾海生,巫繁菁,韦玉燕.紫外分光光度法测定双山颗粒中总黄酮的含量[J].广西科学院学报,2010,26(3):277-279. [点击复制]
- CHEN Yong,WEI Tao,WEI Hou-chao,LU Sen-hua,ZENG Hai-sheng,WU Fan-jing,WEI Yu-yan.Determination of the Total Flavones in Shuangshan Granules[J].Journal of Guangxi Academy of Sciences,2010,26(3):277-279. [点击复制]
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| 摘要: |
| 采用紫外分光光度法,以芦丁为对照品,对其进行络合显色,在500nm波长处测定吸光度,测定双山颗粒剂中总黄酮的含量。结果显示,芦丁对照品的线性范围为8.04~48.24μg/ml(r=0.9996),总黄酮的平均回收率为98.30%(RSD=18.6%,n=6)。紫外分光光度法简便、准确,可以作为双山颗粒剂的质量控制方法。 |
| 关键词: 分光光度法 总黄酮 含量 双山颗粒 |
| DOI： |
| 投稿时间：2010-06-20 |
| 基金项目:广西科技厅科技项目(合同号:桂科产0815010-7G)资助。 |
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| Determination of the Total Flavones in Shuangshan Granules |
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CHEN Yong, WEI Tao, WEI Hou-chao, LU Sen-hua, ZENG Hai-sheng, WU Fan-jing, WEI Yu-yan
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| (Guangxi Traditional Chinese Medical University, Nanning, Guangxi, 530001, China) |
| Abstract: |
| The method for determination of the total flavones in Shangshan granules was established.Rutin was selected as reference material, and the samples were determined at the wavelengh of 500nm.Rutin showed a good linear relationship with absorbance when its concentration was in the range of 8.04~48.24μg/ml(r=0.9996). The average recovery of rutin was 98.30%(RSD=18.6%,n=6).This method is simple, accurate, which can be used for quality control of Shuangshan Granules. |
| Key words: spectrophotometry total flavones content shuangshan granules |
