| 引用本文： |
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张云光,卢安根,莫建光,徐慧.高效液相色谱法测定畜禽肉中磷脂酰胆碱的含量[J].广西科学院学报,2010,26(3):242-244. [点击复制]
- ZHANG Yun-guang,LU An-gen,MO Jian-guang,XU Hui.Determination of Phosphatidyl Choline in Meat by High Performance Liquid Chromatography[J].Journal of Guangxi Academy of Sciences,2010,26(3):242-244. [点击复制]
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| 摘要: |
| 应用高效液相色谱法(HPLC)测定畜禽肉中磷脂酰胆碱含量。以Intertsil SIL硅胶柱(4.6mm×250mm,5μm)为分离柱,甲醇:水(85:15,V:V)为流动相等度洗脱,流速1.5ml/min,柱温为室温,用紫外检测器在206nm处对磷脂酰胆碱进行测定。测得磷脂酰胆碱在0.5~1.92 mg/ml范围内线性关系良好(r2=0.9957),方法检测限为4.14μg/g,精密度为0.49%(n=5),回收率为89%~102%。该方法灵敏度高,重现性和稳定性比较好。 |
| 关键词: 高效液相色谱法 畜禽肉 磷脂酰胆碱 含量测定 |
| DOI： |
| 投稿时间：2010-05-12 |
| 基金项目:广西大型仪器协作共用网资助课题。 |
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| Determination of Phosphatidyl Choline in Meat by High Performance Liquid Chromatography |
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ZHANG Yun-guang1, LU An-gen2, MO Jian-guang2, XU Hui2
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| (1.Nanning Bioclone Biotechnology Co., Ltd., Nanning, Guangxi, 530003, China;2.Guangxi Research Center of Analysis and Testing, Nanning, Guangxi, 530022, China) |
| Abstract: |
| The phosphatidyl choline(PC) in meat is determinated using high performance liquid chromatography(HPLC).Phosphatidyl choline was successfully separated on a Intertsil SIL column (4.6mm×250mm,5μm) using methanol-water(85:15,V:V) as mobile phase at a flow rate of 1.5ml/min and the drift tube temperature is room-temperature.The linear detection range of PC was 0.5~1.92 mg/ml (r2=0.9957) and the limit of etection(LOD) was 4.14μg/g with excellent relative standard deviation (RSD) of 0.49% (n=6).The recovery of PC was in the range of 89%~102%.These results indicated that this method was a simple and fast method with more accuracy and reproduciblity to detect phosphatidyl choline. |
| Key words: HPLC meat phosphatidyl choline quanlity determination |
