| 引用本文： |
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杜寒春,徐慧,黄岛平,陈建红,蒋艳芳.高效液相色谱法测定土壤中的多环芳烃[J].广西科学院学报,2010,26(3):245-247,251. [点击复制]
- DU Han-chun,XU Hui,HUANG Dao-ping,CHEN Jian-hong,JIANG Yan-fang.Determination of Polycyclic Aromatic Hydrocarbons in Soil by High Performance Liquid Chromatography[J].Journal of Guangxi Academy of Sciences,2010,26(3):245-247,251. [点击复制]
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| 摘要: |
| 采用高效液相色谱-荧光检测器快速测定土壤中15种多环芳烃。通过对液相色谱柱、梯度洗脱程序、检测波长程序及柱温等液相色谱条件的选择和优化,使土壤样品中15种多环芳烃在32min内得到完全分离,方法的最低检出限为0.0002~0.0045 mg/kg,回收率为71.20%~95.07%,RSD为5%~10%。该优化方法的精密度高、检出限低、重复性好,是一种能够快速、准确测定土壤中多环芳烃的检测方法。 |
| 关键词: 高效液相色谱法 多环芳烃 土壤 |
| DOI： |
| 投稿时间：2010-06-20 |
| 基金项目:广西科技基础条件平台建设项目(合同编号:08-05-01D)资助。 |
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| Determination of Polycyclic Aromatic Hydrocarbons in Soil by High Performance Liquid Chromatography |
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DU Han-chun, XU Hui, HUANG Dao-ping, CHEN Jian-hong, JIANG Yan-fang
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| (Guangxi Research Center of Analysis and Testing, Nanning, Guangxi, 530022, China) |
| Abstract: |
| Fluorescence detection-high performance liquid chromatographic method was applied to rapidly determine 15 polycyclic aromatic hydrocarbons(PAHs) in soil. The method realized the complete separation of 15 PAHs in 32min by selecting and optimizing the chromatographic conditions,including liquid chromatography column,the gradient elution procedure,the detection wavelength program and the column temperature. The detection limits and the recoveries of the improved method for the 15 PAHs were in the ranges of 0.0002mg/kg to 0.0045mg/kg and 71.20% to 95.07%,respectively.The relative standard deviations (RSD) of this method were in the range of 5% to 10%.The results indicated that this method is a rapid and accurate method with high precision,low detection limit and good reproducibility for the determination of PAHs in soil. |
| Key words: high performance liquid chromatography (HPLC) polycyclic aromatic hydrocarbons (PAHs) soil |
