| 摘要: |
| 以甲醇为溶剂,用索式提取法提取蓝耳草[Cyanotis vaga(Lour.) Roem.et Schult]中β-蜕皮激素,用高效液相色谱法(HPLC)测定其含量。结果,β-蜕皮激素在0.4~8.0μg范围内线性关系良好,r2=0.9992,平均回收率为99.31%,RSD值均小于3%。 |
| 关键词: 高效液相色谱法 β-蜕皮激素 蓝耳草 提取工艺 |
| DOI： |
| 投稿时间：2010-05-11 |
| 基金项目: |
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| Determination of β-Ecdysone in Cyanotis vaga (Lour.) Roem.et Schult by HPLC |
|
XU Hui
|
| (Guangxi Research Center of Analysis and Testing, Nanning, Guangxi, 530022, China) |
| Abstract: |
| Using methanol as the solvent extraction rate,the β-Ecdysone in Cyanotis vaga (Lour.) Roemet Schult is extracted by the soxhlet extraction method.The content of β-Ecdysone is determined by HPLC.A linear curve of β-Ecdysone was obtained in the range of 0.4~0.8μg with r2 equalling to 0.9992.The average recovery was 99.31% with RSD being less than 3%. |
| Key words: HPLC β-Ecdysone Cyanotis vaga(Lour.) Roem.et Schult extraction process |
