| 引用本文： |
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李晓静,陈桂鸾,徐远金.胶束电动毛细管色谱法分析性激素[J].广西科学院学报,2006,22(S1):463-465. [点击复制]
- LI Xiao-jing,CHEN Gui-luan,XU Yuan-jin.Determination of Sex Hormones by Capillary Micellar Electrokinetic Chromatography[J].Journal of Guangxi Academy of Sciences,2006,22(S1):463-465. [点击复制]
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| 摘要: |
| 用胶束电动毛细管色谱法分离和测定雌三醇、炔诺酮、睾酮、雌酮、雌二醇、炔雌醇、黄体酮性激素,考察各种操作参数及有机添加剂对分离的影响。各组分在7min内得到很好的分离,检出限1.0~4.5 mg/L,样品的加标回收率91.5~106.3 mg/L,相对标准偏差4.7%~5.3%。该方法简便、快速,已成功用于炔诺酮片和鹿鞭提取液中性激素的同时分析。 |
| 关键词: 胶束电动毛细管色谱法 性激素 炔诺酮片 鹿鞭 |
| DOI： |
| 投稿时间：2006-07-25 |
| 基金项目: |
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| Determination of Sex Hormones by Capillary Micellar Electrokinetic Chromatography |
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LI Xiao-jing1, CHEN Gui-luan1, XU Yuan-jin1,2
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| (1.Engineering Center for Sugar Cane and Cane Sugar, Guangxi University, Nanning, Guangxi, 530004, China;2.Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization, Guangxi University, Nanning, Guangxi, 530004, China) |
| Abstract: |
| A micellar electrokinetic capillary chromatography (MEKC) separation of seven sex hormones,estriol,norethisterone,testosterone,estrone,β-estradiol,ethynylestradiol and progesterone,was developed with a micellar solution consisting of 30 mmol/L sodium dodecyl sulphate(SDS),20 mmol/L H3BO3,15 mmol/L Na2HPO4 and 15%(v/v) isopropyl alcohol(pH was adjusted to 9.0 with NaOH).Results showed that satisfactory separation was achieved within 7 minutes.The detection limits,average recoveries and relative standard deviations are 1.04.5 mg/L,91.5106.3 mg/L and 4.7%~5.3%,respectively.The method is simple,rapid and suitable for the simultaneous determination of the seven sex hormones in norethisterone tablets and Penis et Testis Cervi. |
| Key words: micellar electrokinetic chromatography sex hormones norethisterone tablets penis et testis cervi |
