| 摘要: |
| 导数光谱是紫外可见分光光度法中用以排除干扰的一种技术。本文用日立220-A紫外可见分光光度计中测定了血清蛋白质的含量。通过实验得出的线性回归方程为D=155.24c-0.346;相关系数r=0.9998,样本平均回收率为100.05%,精度试验d=0.077,S=0.418,dx=0.027,Sx=0.148。此法重现好,变异系数(CV)<±1%,此法简单,快速,可用于血清蛋白质的测定。 |
| 关键词: 导数光谱法 蛋白质 |
| DOI： |
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| 基金项目: |
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| DETERMINATION OF SERUM PROTEIN BY ULTRAVIDET SECONDARY DERIVATIVE SPECTROSCOPY |
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Qin Zhijian
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| (A Central Laboratory of a National Madical College of the youjiang Guangxi, Borse) |
| Abstract: |
| The derivative Spectrometry is a technique for eliminating the interference in ultraviole-visible spectra.This paper deals with the determination of content of protein by the second derivation spectrometry with uv-220-A spectrophotometer. under the Sxperimental condition the linear equation D=155.24c-0.346,the correlation cofficient r=0.9998, the arerage recovery ratio of semple is 100.05%.in serum.The precise test d=0.077, S=0.418, dx=0.027, Sx=0.148. the repeatatability is good and relative deviation is less than卤1%, the method is simple end rapid. the method can be used in the determination of content of protein in serum. |
| Key words: Derivative spectrometry Protein |
