| 引用本文： |
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甘雨满,黄炳耀,唐振洲,林霄,高程海.二十四元大环内酯生物合成研究进展[J].广西科学,2020,27(5):487-492. [点击复制]
- GAN Yuman,HUANG Bingyao,TANG Zhenzhou,LIN Xiao,GAO Chenghai.Research Progress in Macrolactins Bio-synthesis[J].Guangxi Sciences,2020,27(5):487-492. [点击复制]
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| 摘要: |
| 二十四元大环内酯Macrolatins对大部分革兰氏阳性菌和阴性菌具有显著抑制活性，在疾病治疗、农业病虫害防治等领域具有潜在的应用价值。但野生菌株代谢产生的二十四元大环内酯产量较低，不利于该类化合物的进一步研究。基于此，本文介绍了二十四元大环内酯生物合成基因簇、基因簇重构、工程菌株构建和发酵优化方面取得的研究成果，简要分析二十四元大环内酯生物合成所面临困境以及下一步研究应该重点关注的方向，为其进一步研究和开发利用提供参考依据。 |
| 关键词: 二十四元大环内酯 PKS基因簇 工程菌株 发酵优化 生物合成 |
| DOI：10.13656/j.cnki.gxkx.20201214.012 |
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| 基金项目:国家自然科学基金项目（31960019），广西自然科学基金项目（2019GXNSFBA185023，2018GXNSFAA281268），广西中医药大学博士启动基金项目（2018BS044，2017BS039），广西中医药大学岐黄工程高层次人才团队培育项目（2018006）和广西八桂学者专项（05019055）资助。 |
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| Research Progress in Macrolactins Bio-synthesis |
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GAN Yuman, HUANG Bingyao, TANG Zhenzhou, LIN Xiao, GAO Chenghai
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| (Institute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning, Guangxi, 530200, China) |
| Abstract: |
| Macrolactins show significant inhibitory activity on most of gram-positive and gram-negative bacteria,and has potential application value in the fields of disease treatment and agricultural pest control.However,the content of macrolactins obtained from wild-type strains is relatively low,which is not conducive to further research on this type of compound.Based on this,this article introduces the research results of macrolactins biosynthesis gene cluster,gene cluster recombination,engineering strain construction and fermentation optimization.The difficulties in biosynthesis of macrolactins and the direction that the next research should focus on are also analyzed,which will provide a reference for its further research and development. |
| Key words: macrolactins PKS gene cluster MLNs engineering strain fermentation optimization |
