| 引用本文： |
-
张国艳,张欢欢,员君华,杨苗苗,袁娇,郭明明,邹樊,周志成,李雯,齐向辉.基因重组型枯草芽孢杆菌芽孢表面展示技术与应用[J].广西科学,2018,25(3):242-247,257. [点击复制]
- ZHANG Guoyan,ZHANG Huanhuan,YUN Junhua,YANG Miaomiao,YUAN Jiao,GUO Mingming,ZOU Fan,ZHOU Zhicheng,LI Wen,QI Xianghui.Technique and Application of Recombinant Bacillus subtilis Spore Surface Display[J].Guangxi Sciences,2018,25(3):242-247,257. [点击复制]
|
|
| |
|
|
| 本文已被：浏览 934次 下载 847次 |
码上扫一扫！ |
| 基因重组型枯草芽孢杆菌芽孢表面展示技术与应用 |
|
张国艳, 张欢欢, 员君华, 杨苗苗, 袁娇, 郭明明, 邹樊, 周志成, 李雯, 齐向辉
|
|
|
| (江苏大学, 食品与生物工程学院, 江苏镇江 212013) |
|
| 摘要: |
| 枯草芽孢杆菌是一种好氧型革兰氏阳性益生菌,在营养匮乏的环境下,可形成具有强抗逆性的芽孢。其芽孢独特的结构和生理功能使得芽孢表面展示技术相较于其他表面展示技术具有多种优势,构建的重组芽孢不但易纯化、回收率高,而且安全性好。近年来,枯草芽孢杆菌芽孢表面展示技术得到迅猛发展,已成功利用CotB、CotC、CotG、CotZ、CotA、OxdD、CotE、CotZ、CgeA等多种锚定蛋白将外源蛋白或多肽展示在芽孢表面,并应用于工业化酶、口服疫苗和药物、大分子量多聚体蛋白的生产以及环境污染的生物治理等领域。本文首先介绍了芽孢展示系统整合策略,并重点阐述了基因重组型枯草芽孢杆菌芽孢展示技术中涉及的锚定蛋白以及该技术在各领域的应用与展望。 |
| 关键词: 枯草芽孢杆菌 芽孢表面展示 基因重组 锚定蛋白应用 |
| DOI：10.13656/j.cnki.gxkx.20180522.001 |
| 投稿时间：2018-04-21 |
| 基金项目:国家重点研发计划(2017YFC1600806),江苏省"六大人才高峰"项目(SWYY-018),国家自然科学基金项目(31571806)和中国博士后基金项目(2017M621657)资助 |
|
| Technique and Application of Recombinant Bacillus subtilis Spore Surface Display |
|
ZHANG Guoyan, ZHANG Huanhuan, YUN Junhua, YANG Miaomiao, YUAN Jiao, GUO Mingming, ZOU Fan, ZHOU Zhicheng, LI Wen, QI Xianghui
|
| (School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu, 212013, China) |
| Abstract: |
| Bacillus subtilis is an aerobic, gram-positive probiotic, which can form spores on strong stress resistant when facing the nutritional starvation.Owing to the unique structure and physiological function of spores, spore surface display technologies have many advantages over other ones.The constructed recombinant spores are not only easy to purify, but also have high recovery rate and good safety.Bacillus subtilis surface display technique has been developed rapidly in recent years.In recent years, Bacillus subtilis spore surface display technology has been rapidly developed, and various anchored proteins such as CotB, CotC, CotG, CotZ, CotA, OxdD, CotE, CotZ, and CgeA have been successfully used to display foreign proteins or polypeptides on spore surfaces and applied to the fields of industrial enzymes, oral vaccines and drugs, the production of large molecular weight polymer protein and biological treatment of environmental pollution and other fields.In this study, the integration strategy of the spore display system is introduced firstly, and the applications of anchored proteins in Bacillus subtilis spores display technology, as well as its application and prospect in various fields are expounded further. |
| Key words: Bacillus subtilis spore surface display gene recombination anchor protein application |
|
|
|
|
|
