引用本文
  • 熊海涛,韦宇拓.枯草芽孢杆菌表达系统及其启动子的研究进展[J].广西科学,2018,25(3):233-241.    [点击复制]
  • XIONG Haitao,WEI Yutuo.Research Progress of Bacillus subtilis Expression System and Its Promoter Regulatory Elements[J].Guangxi Sciences,2018,25(3):233-241.   [点击复制]
【打印本页】 【在线阅读全文】【下载PDF全文】 查看/发表评论下载PDF阅读器关闭

←前一篇|后一篇→

过刊浏览    高级检索

本文已被:浏览 327次   下载 787 本文二维码信息
码上扫一扫!
枯草芽孢杆菌表达系统及其启动子的研究进展
熊海涛1,2, 韦宇拓1,2
0
(1.广西大学生命科学与技术学院, 广西南宁 530005;2.亚热带农业生物资源保护与利用国家重点实验室, 广西南宁 530005)
摘要:
枯草芽孢杆菌(Bacillus subtilis)是工业应用中一种重要的模式菌株,具有非致病性、遗传背景清晰、分泌蛋白能力强、容易分离培养等特性,是异源蛋白表达和分泌的理想宿主。高效可控的启动子是实现外源蛋白高效表达的关键因素之一。根据诱导机制,启动子可分为组成型启动子、诱导型启动子、自诱导启动子和时期特异性启动子。本文介绍了枯草芽孢杆菌表达系统、芽孢杆菌σ因子类型及枯草芽孢杆菌启动子的结构,比较了常用启动子的优缺点,总结了新启动子克隆和改造及生物信息学预测启动子的方法,并对枯草芽孢杆菌启动子未来的研究方向进行展望,为进一步研究启动子的结构和功能及工业生产外源蛋白奠定基础。
关键词:  枯草芽孢杆菌  表达系统  启动子
DOI:10.13656/j.cnki.gxkx.20180528.001
投稿时间:2018-05-08
基金项目:广西自治区主席科技资金项目(NO:16449-01)资助
Research Progress of Bacillus subtilis Expression System and Its Promoter Regulatory Elements
XIONG Haitao1,2, WEI Yutuo1,2
(1.College of Science and Technology, Guangxi University, Naning, Guangxi, 530005, China;2.State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Nanning, Guangxi, 530005, China)
Abstract:
Bacillus subtilis is an important model strain in industrial application.It has the characteristics of non-pathogenicity, clear genetic background, strong protein secretion ability, easy isolation and culture.It is an ideal host for heterologous protein expression and secretion.Efficient and controllable promoters are one of the key factors for the efficient expression of heterologous proteins.According to the induction mechanism, promoters can be divided into constitutive promoters, inducible promoters, auto-inducible promoters and stage specific promoters.This article briefly introduced the Bacillus subtilis expression system, Bacillus sigma factor type and Bacillus subtilis promoter structure, compared the advantages and disadvantages of commonly used promoters, summarized the methods for the cloning and transformation and bioinformatics prediction of new promoters, prospected the future research direction of the Bacillus subtilis promoter which laid the foundation for further research on the structure and function of promoters and industrial production of foreign aid proteins.
Key words:  Bacillus subtilis  expression system  promoter

用微信扫一扫

用微信扫一扫