| 摘要: |
| 丝状真菌是重要的工业酶生产菌,具有优异的蛋白表达和分泌能力,如里氏木霉在工业发酵中其内源纤维素酶产量可高达100 g/L。目前,丝状真菌已被成功用于表达外源基因,但与内源基因相比,外源基因的表达效率常常不高。使用强启动子来驱动基因转录可有效提高外源基因的表达水平,因此本文就通过基因工程的方法对丝状真菌的启动子进行改造以提高内、外源基因的转录效率的研究进行总结与展望。 |
| 关键词: 丝状真菌 启动子 基因工程 |
| DOI：10.13656/j.cnki.gxkx.20170223.002 |
| 投稿时间：2017-01-15修订日期：2017-02-18 |
| 基金项目:国家自然科学基金青年基金项目"多基因协同调控里氏木霉高效分泌表达蛋白的研究"(项目批准号:31400067)资助 |
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| Research Progress Towards Promoters Genetic Engineering in Filamentous Fungi |
|
SUN Xianhua, SU Xiaoyun
|
| (Institute of Feed Research of CAAS, Beijing, 100081, China) |
| Abstract: |
| Filamentous fungi are important industrial microbes with excellent ability of protein expression and secretion.For example, Trichoderma reesei can produce its endogenouscellulase with a titer of up to 100 g/L in industrial fermentation conditions.Currently, filamentous fungi have been successfully used to express heterologous genes.However, compared with endogenous gene expression, the expression of heterologous genes in filamentous fungi is often of low efficiency.In order to increase the expression level of heterologous genes, one effective way is to use strong promoters to drive gene transcription.In this paper, we discuss the progress of genetic modification of the filamentous fungi promoters to improve transcription efficiency of endogenous and heterologous genes. |
| Key words: filamentous fungi promoter genetic engineering |
