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李文琪,陈明生,温幼敏,马军花,陈露,黄艳,刘布鸣.光石韦药材中芒果苷的定性定量分析方法研究[J].广西科学,2012,19(1):77-79. [点击复制]
- LI Wen-qi,CHEN Ming-sheng,WEN You-min,MA Jun-hua,CHEN Lu,HUANG Yan,LIU Bu-ming.Qualitative and Quantitative Analysis of Mangiferin in Pyrrosia hclavata (Bak.) Ching[J].Guangxi Sciences,2012,19(1):77-79. [点击复制]
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| 光石韦药材中芒果苷的定性定量分析方法研究 |
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李文琪1,2, 陈明生1,2, 温幼敏3, 马军花3, 陈露1,2, 黄艳1,2, 刘布鸣1,2
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| (1.广西中药质量标准研究重点实验室, 广西南宁 530022;2.广西中医药研究院, 广西南宁 530022;3.广西万通制药有限公司, 广西南宁 530003) |
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| 摘要: |
| 为了研究光石韦药材中的芒果苷,建立定性定量分析方法,采用薄层色谱定性鉴别,高效液相色谱测定芒果苷含量。结果表明,薄层色谱鉴别方法的重现性好,芒果苷在0.0605~0.3632 μg范围内呈良好线性关系(r=0.9996),平均回收率为101.3%,RSD为2.13%(n=9)。方法简便、准确,专属性强,重现性和回收率好,可以为光石韦药材的质量标准制定提供分析方法和依据。 |
| 关键词: 芒果苷 薄层色谱 高效液相色谱 光石韦 |
| DOI： |
| 投稿时间：2012-01-31修订日期：2012-02-11 |
| 基金项目:广西科技攻关项目(桂科攻1099062-4)和南宁技术创新项目(2009-2-15)资助。 |
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| Qualitative and Quantitative Analysis of Mangiferin in Pyrrosia hclavata (Bak.) Ching |
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LI Wen-qi1,2, CHEN Ming-sheng1,2, WEN You-min3, MA Jun-hua3, CHEN Lu1,2, HUANG Yan1,2, LIU Bu-ming1,2
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| (1.Guangxi Key Laboratory of Traditional Chinese Medicine Quality Stardands, Nanning, Guangxi, 530022, China;2.Guangxi Institute of Traditional Medical and Pharmaceutical Sciences, Nanning, Guangxi, 530022, China;3.Wantong Pharmaceutical Co., Ltd., Guangxi, Nanning, Guangxi, 530003, China) |
| Abstract: |
| TLC and HPLC were adopted to establish qualitative and quantitative determination of mangiferin in Pyrrosia clavata(Bak.) Ching. The results showed that the qualitative method was repeatable, the calibration curve of mangiferin was linear within the range of 0.0605~0.3632μg, r=0.9996. Average recovery was 101.3% and RSD was 2.13% (n=6).This method is accuracy, sensitive and simple, and can be adopted as a standard for the quality control of Pyrrosia clavata(Bak.) Ching. |
| Key words: mangiferin TLC HPLC Pyrrosia clavata(Bak.) Ching |
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