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陈明生,黄艳,林霄,柴玲,稂文旺,李齐修,刘布鸣.高效液相色谱法测定互叶白千层中没食子酸的含量[J].广西科学,2012,19(1):71-73. [点击复制]
- CHEN Ming-sheng,HUANG Yan,LIN Xiao,CHAI Ling,LANG Wen-wang,LI Qi-xiu,LIU Bu-ming.Determination of Gallic Acid in Melaleuca alternifolia by HPLC[J].Guangxi Sciences,2012,19(1):71-73. [点击复制]
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| 高效液相色谱法测定互叶白千层中没食子酸的含量 |
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陈明生1,2, 黄艳1,2, 林霄1,2, 柴玲1,2, 稂文旺1,2, 李齐修1,2, 刘布鸣1,2
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| (1.广西中药质量标准研究重点实验室, 广西南宁 530022;2.广西壮族自治区中医药研究院, 广西南宁 530022) |
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| 摘要: |
| 为了建立用高效液相色谱测定互叶白千层中没食子酸含量的方法,为互叶白千层的质量标准制定提供方法和依据,采用高效液相色谱法(HPLC)测定互叶白千层中没食子酸含量。色谱柱为Diamonsil C18(4.6mm×250mm,5μm),流动相为乙腈-0.2%磷酸(3:97),检测波长274nm,柱温为室温,流速1ml/min。结果,没食子酸在0.2016~1.2096μg范围内呈良好线性关系(r=0.9991),平均回收率为101.87%,RSD为1.76%(n=9)。本方法简便可行,重现性好,可以作为互叶白千层中没食子酸含量的测定方法。 |
| 关键词: 没食子酸 高效液相色谱 含量测定 互叶白千层 |
| DOI： |
| 投稿时间：2011-11-17 |
| 基金项目:广西科学基金项目(2010GXNSFB013085)资助。 |
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| Determination of Gallic Acid in Melaleuca alternifolia by HPLC |
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CHEN Ming-sheng1,2, HUANG Yan1,2, LIN Xiao1,2, CHAI Ling1,2, LANG Wen-wang1,2, LI Qi-xiu1,2, LIU Bu-ming1,2
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| (1.Guangxi Key Laboratory of Traditional Chinese Medicine Quality Stardands, Nanning, Guangxi, 530022, China;2.Guangxi Institute of Traditional Medical and Pharmaceutical Sciences, Nanning, Guangxi, 530022, China) |
| Abstract: |
| A high-performance liquid chromatography(HPLC) method was developed for the determination of gallic acid in Melaleuca alternifolia, which provided the basis for establishing the quality standard of Melaleuca alternifolia.The determination was performed on Diamonsil C18(4.6mm×250mm, 5μm) column with acetonitrile-0.2% phosphoric acid(3:97) as mobile phase with flow rate of 1.0 ml/min at detection wavelength of 274 nm.And the column temperature was set at room temperature.The average recovery rate(n=9) of gallic acid was 101.87% and RSD was 1.76%.This method is simple, rapid, accurate and reliable, and is suitable for the quality control of gallic acid in Melaleuca alternifolia. |
| Key words: gallic acid HPLC determination Melaleuca alternifolia (Maiden et Betehe) Chee1 |
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