| 引用本文： |
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杨登峰,关妮,米慧芝,杜奇石,张穗生,黄日波.眉斑并脊天牛纤维素酶性质的研究[J].广西科学,2011,18(3):261-263,268. [点击复制]
- YANG Deng-feng,GUAN Ni,MI Hui-zhi,DU Qi-shi,ZHANG Sui-sheng,HUANG Ri-bo.Research on Glenea cantor Cellulase Characteristics[J].Guangxi Sciences,2011,18(3):261-263,268. [点击复制]
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| 摘要: |
| 为了了解眉斑并脊天牛(Glenea cantor)纤维素酶的性质,选取以木棉树(Bombax ceiba)为食的眉斑并脊天牛为研究对象,按照IUPAC的标准测定方法,测定和定位眉斑并脊天牛肠道中的内切-β-1,4葡聚糖酶、纤维二糖酶以及滤纸酶活力,考察内切-β-1,4葡聚糖酶的最适pH值和最适温度。结果表明:在眉斑并脊天牛肠道中同时存在以上3种纤维素酶,3种纤维素酶都具有酶活力,并且主要集中在天牛肠道的中肠。内切-β-1,4葡聚糖酶具有广泛的pH值和温度适应范围,最适pH值为9.2,最适温度为50℃。 |
| 关键词: 眉斑并脊天牛 纤维素 纤维素酶 |
| DOI： |
| 投稿时间：2011-01-07 |
| 基金项目:广西科学基金项目(桂科青0832016)资助 |
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| Research on Glenea cantor Cellulase Characteristics |
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YANG Deng-feng, GUAN Ni, MI Hui-zhi, DU Qi-shi, ZHANG Sui-sheng, HUANG Ri-bo
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| (State Key Laboratory of Non-Food Biomass and Enzyme Technology, National Engineering Research Center for Non-food Biorefinery, Guangxi Key Laboratory of Biorefinery, Guangxi Academy of Sciences, Nanning, Guangxi, 530007, China) |
| Abstract: |
| According to the standard method from IUPAC, the activities of endo-β-1, 4-glucanase, cellobiase and filter paper enzyme from Glenea cantor were analyzed and their locations were determined.Optimal pH value and optimal temperature of endo-β-1, 4-glucanase were measured.The results showed that there were activities of all above three cellulases in Glenea cantor.They were mainly located in the midgut.The endo-β-1, 4-glucanase had activity in a broad pH value and temperature range.Optimal pH value and optimal temperature of the endo-β-1, 4-glucanase were 9.2 and 50℃, respectively. |
| Key words: Glenea cantor cellulose cellulase |
