| 引用本文： |
-
陈小玲,陈东,芦志龙.瑞氏木霉内切-β-1,4葡聚糖酶基因Egl1的分子改造[J].广西科学,2011,18(3):264-268. [点击复制]
- CHEN Xiao-ling,CHEN Dong,LU Zhi-long.Molecular Modification of Endo-β-1, 4-glucanase Gene Egl1 from Trichoderma reesei[J].Guangxi Sciences,2011,18(3):264-268. [点击复制]
|
|
| 摘要: |
| 为了提高瑞氏木霉(Trichoderma reesei)的纤维素酶活力,用分子改造的方法改造其内切-β-1,4葡聚糖酶基因Egl1。用DNaseⅠ消化Egl1,回收50~200bp的片段,用T4 DNA连接酶连接回收的片段,进行PCR反应,将PCR产物转入瑞氏木霉原生质体,用比较滤纸酶活力的方法筛选纤维素酶活力提高的菌株。结果筛选到2株滤纸酶活力比出发菌株提高的菌株Egl3 6-4和Egl3 3-4B2Z2,其酶活力分别比出发菌株提高了2.8倍和3.6倍。 |
| 关键词: 瑞氏木霉 纤维素酶 分子改造 |
| DOI： |
| 投稿时间：2011-01-07 |
| 基金项目:广西科技攻关项目(桂科转10123005-17);广西科学院基本科研业务费项目(10YJ25SW12)资助 |
|
| Molecular Modification of Endo-β-1, 4-glucanase Gene Egl1 from Trichoderma reesei |
|
CHEN Xiao-ling, CHEN Dong, LU Zhi-long
|
| (State Key Laboratory of Non-food Biomass Enzyme Technology, National Engineering Research Center for Non-food Biorefinery, Guangxi Key Laboratory of Biorefinery, Guangxi Academy of Sciences, Nanning, Guangxi, 530007, China) |
| Abstract: |
| Endo-β-1, 4-glucanase gene Egl1 was digested with DNase Ⅰ, fragments of 50~200bp were collected and randomly linked with T4 DNA, then were used as templates for sequences amplification by polymerase chain reaction (PCR).The shuffling sequences were transformed to T.reesei by protoplast transformation.Two mutants with higher cellulase activity were selected.The filter paper activity (FPA) of two mutants named Egl3 6-4 and Egl3 3-4B2Z2 were 2.8 and 3.6 times higher than that of original strain, respectively. |
| Key words: Trichoderma reesei cellulase molecular modification shuffling |
