| 引用本文： |
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郑元涛,彭立新,姚辉璐,黄庶识,王桂文.FTIR-HATR诊断α-地中海贫血[J].广西科学,2011,18(3):228-232. [点击复制]
- ZHENG Yuan-tao,PENG Li-xin,YAO Hui-lu,HUANG Su-shi,WANG Gui-wen.Identification of α-Thalassemia by FTIR-HATR Spectroscopy[J].Guangxi Sciences,2011,18(3):228-232. [点击复制]
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| 摘要: |
| 将傅里叶红外光谱与水平衰减全反射技术(FTIR-HATR)结合,对α-地中海贫血样品与正常样品进行检验,通过改进的相对强度计算方法进行对比分析。结果表明:α-地贫组与正常组间在I1074/I1152、I1517/I1074、I3295/I3372相对强度上存在显著差异;在峰的指认上,波数1074cm-1、1152cm-1、1517cm-1和3295cm-1分别表征磷酸类化合物、碳水化合物、酪氨酸和Amide A,其相对强度差异应主要来源于细胞内增加的高磷化合物2,3-二磷酸甘油酸、减少的葡萄糖以及蛋白组分;由于以上组分的变化被相对强度计算所放大,在组间呈显著的特异性,通过相对强度与主成分析均能将混合的样品正确区分。 |
| 关键词: 血红蛋白 α-地中海贫血 FTIR-HATR 相对强度 |
| DOI： |
| 投稿时间：2011-01-07 |
| 基金项目:国家自然科学基金项目(No.60868002);广西科学基金项目(No.0832022Z)资助 |
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| Identification of α-Thalassemia by FTIR-HATR Spectroscopy |
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ZHENG Yuan-tao1, PENG Li-xin2, YAO Hui-lu3, HUANG Su-shi3, WANG Gui-wen3
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| (1.Biology Institute, Guangxi Academy of Sciences, Nanning, Guangxi, 530007, China;2.State Key Laboratory of Non-food Biomass Enzyme Technology, National Engineering Research Center for Non-food Biorefinery, Guangxi Key Laboratory of Biorefinery, Guangxi Academy of Sciences, Nanning, Guangxi, 530007, China;3.Biophysics Laboratory, Guangxi Academy of Sciences, Nanning, Guangxi, 530007, China) |
| Abstract: |
| FTIR associated with multi-bounce horizontal attenuated total reflectance was used to diagnose α-thalassemia patients.In ranges of 800~1780cm-1 and 2480~3600cm-1, we calculated all intensity ratios to detect spectra differences between patient and health groups.We found that there are significant differences between α-thalassemia patients and health groups in intensity ratios of I1074/I1152, I1517/I1074 and I3295/I3372.At the peaks of 1074cm-1, 1152cm-1, 1517cm-1 and 3295cm-1, they are assigned to deformation vibration of O-P=O, C-O stretching vibration of carbohydrate, breathing vibration of tyrosine ring and Amide A, respectively.It suggests that the difference of intensity ratios between two groups may result from increasing 2, 3-diphosphoglycerate, decreasing glucose and hemoglobin in α-thalassemia patients.Furthermore, principal components analysis (PCA) was used to assess these ratios and demonstrated a correct classification on the basis of PC scores. |
| Key words: hemoglobin α-thalassemia FTIR-HATR intensity ratios |
