| 引用本文： |
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陶卓奇,田红艳,韦向玲,梁情,潘灵娟,李芳芳,廖森.铬天青Cu(Ⅱ)配合物与牛血清白蛋白作用的光谱研究[J].广西科学,2010,17(1):68-71. [点击复制]
- TAO Zhuo-qi,TIAN Hong-yan,WEI Xiang-ling,LIANG Qing,PAN Ling-juan,LI Fang-fang,LIAO Sen.Spectra Study on the Interaction Between the Complexes of CAS-Cu (Ⅱ) and Bovine Serum Albumin[J].Guangxi Sciences,2010,17(1):68-71. [点击复制]
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| 摘要: |
| 结合均匀设计试验方法及数据挖掘技术,采用紫外-可见吸收光谱及共振散射光谱研究铬天青Cu(Ⅱ)配合物与牛血清白蛋白的相互作用。确定试验的最佳条件为pH值=6,0.1mol/L离子强度剂(NaCl)用量0.5ml,0.1%铬天青用量0.6ml。得到紫外-可见吸收光谱的吸光度随着铜离子浓度的增加而线性下降,其对应的方程及参数为:ΔA=0.08302-0.06207C,R=-0.9987,SD=0.001830。共振散射光谱的强度则随着铜离子浓度的增加而线性的增强,其对应的方程及参数为:ΔI=-226.15+287.9C,R=0.9866,SD=17.38。 |
| 关键词: 配合物,铬天青Cu(Ⅱ) 牛血清白蛋白 紫外-可见吸收光谱 共振散射光谱 均匀设计 数据挖掘 |
| DOI： |
| 投稿时间：2009-04-18修订日期：2009-05-18 |
| 基金项目:教育部科研重点项目(批准号:205120)和广西科学基金(批准号:桂科基0575006,桂科自0640009),广西大学化学化工学院创新实践基金(2007年)资助。 |
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| Spectra Study on the Interaction Between the Complexes of CAS-Cu (Ⅱ) and Bovine Serum Albumin |
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TAO Zhuo-qi, TIAN Hong-yan, WEI Xiang-ling, LIANG Qing, PAN Ling-juan, LI Fang-fang, LIAO Sen
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| (School of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi, 530004, China) |
| Abstract: |
| The interaction between CAS-Cu (Ⅱ) complex and bovine serum albumin (BSA) was studied with UV absorption spectroscopy and resonance scattering spectroscopy.Uniform experimental design as well as data mining technology were applied in the experiments, in which the optimal technical conditions were obtained. The experimental results of the optimal technical conditions showed that the absorbency of UV absorption spectra linearly decreased with increase of Cu (Ⅱ) concentration.The linear equation and parameters were:ΔA=0.08302-0.06207C, R=-0.9987, SD=0.001830, and the intensity of resonance scattering spectra linearly increased with increase of Cu (Ⅱ) concentration.The linear equation and parameters were:ΔI=-226.148+287.9015C, R=0.9866, SD=17.38261. |
| Key words: complex CAS-Cu(Ⅱ) bovine serum albumin (BSA) electron spectroscopy resonance scattering spectroscopy uniform design data mining |
