| 摘要: |
| 在硼酸缓冲液和In(Ⅲ)-HQS荧光络合体系中加入一定浓度的半胱氨酸,体系的荧光强度与半胱氨酸的加入量成正比,从而提出一种新的荧光光度法测定半胱氨酸。该方法测定半胱氨酸的检出限为15ng·ml-1(SNR=3),大多数的氨基酸即使浓度较高时也无干扰。用该方法测定胱氨酸电解液中的半胱氨酸回收率为95.7%~101.3%。 |
| 关键词: 半胱氨酸 荧光光度法 In (Ⅲ)-8-羟基喹啉-5-磺酸荧光络合体系 |
| DOI： |
| 投稿时间：2007-07-13修订日期：2007-10-23 |
| 基金项目: |
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| Study on a New Complex System of Determination of Cysteine by Spectrofluorimetry |
|
HUANG Zhi-biao1, WANG Wei-sheng2
|
| (1.The Center for Analysis & Test Research of Guangxi, Nanning, Guangxi, 530022, China;2.Shanghai Tobacco Company, Shanghai, 200082, China) |
| Abstract: |
| The addition of cysteine (Cys)to the fluorescence system of In (Ⅲ)8-hydroxyquinoline-5-sulphonic acid complex (In (Ⅲ)-HQS)in H3BO3-Na2B4O7 buffer (pH 8.50)solution led to immediate fluorescence inhibition, which was proportional to their amounts.Based on this finding, a new spectrofluorimetric method for the determination of Cys has been developed.The detection limits was 15ng·mL-1 (SNR=3).Most amino acids had no interference at high concentrations.The proposed method has been applied to the determination of Cys in cysteine electrolyte, with recoveries of 95.7%~101.3%. |
| Key words: cysteine spectrofluorimetry In (Ⅲ)-8-hydroxyquinoline-5-sulphonic acid complex |
