| 引用本文： |
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黄永林,文永新,赵志国,朱廷春.RP-HPLC法测定艾纳香中艾纳香素和二氢黄酮醇含量[J].广西科学,2007,14(2):140-142. [点击复制]
- HUANG Yong-lin,WEN Yong-xin,ZHAO Zhi-guo,ZHU Ting-chun.Determination of Flavanones and Flavanonols in Blumea balsanifera DC.by RP-HPLC[J].Guangxi Sciences,2007,14(2):140-142. [点击复制]
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| 摘要: |
| 采用反相高效液相色谱法测定艾纳香中的艾纳香素和二氢黄酮醇含量。确定色谱条件为:色谱柱ZORBAXXDB-C18(4.6mm×150mm,5μm),流动相为V(甲醇):V(水)=60:40,流速0.8ml/min,检测波长290nm,柱温为30℃。在此条件下,艾纳香素和二氢黄酮醇与其它组分得到良好的分离,艾纳香素的线性范围为0.0032~0.048μg/μl(r=0.9999),二氢黄酮醇的线性范围为0.0032~0.048μg/μl(r=0.9993);艾纳香素和二氢黄酮醇的精密度RSD(n=5)分别为1.08%和1.26%,平均回收率(n=5)达98.36%和97.86%。 |
| 关键词: 艾纳香 艾纳香素 二氢黄酮醇 反相高效液相色谱法 |
| DOI： |
| 投稿时间：2007-01-08修订日期：2007-03-02 |
| 基金项目:广西自然科学基金(桂科基0448087);广西科技攻关(桂科攻0630002-4B)资助项目 |
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| Determination of Flavanones and Flavanonols in Blumea balsanifera DC.by RP-HPLC |
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HUANG Yong-lin, WEN Yong-xin, ZHAO Zhi-guo, ZHU Ting-chun
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| (Guangxi Institute of Botany, Guilin, Guangxi, 541006, China) |
| Abstract: |
| The methods of determination flavanones and flavanonols in Blumea balsanifera DC.by HPLC was established in this thesis.The following HPLC conditions are the best:column:ZORBAX XDB-C18 (4.6mm×150mm, 5μm);mobile phase:V (methanol):V (water)=60:40;flow rate:0.8ml/min;detection wavelength:290nm;temperature:30℃.Flavanones and flavanonols were separated thoroughly from other components on this condition, the linearity of flavanones was obtained within the range of 0.0032μg/μl and 0.048μg/μl (r=0.9999respectively), the linearity of flavanonols was obtained within the range of 0.0032μg/μl and 0.048μg/μl (r=0.9993 respectively);the RSD of flavanones and flavanonols for exactitude degree were 1.08% and 1.26% respectively (n=5);average recoveries were 98.36% and 97.86% respectively (n=5). |
| Key words: Blumea balsanifera DC. flavanones flavanonols RP-HPLC |
