| 引用本文： |
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车玉琴,沈京连,刁尧,高杰.胰岛素样生长因子-Ⅱ对大鼠脑缺血再灌注损伤后NSE含量变化影响[J].广西科学,2007,14(2):137-139. [点击复制]
- CHE Yu-qin,SHEN Jing-lian,DIAO Yao,GAO Jie.Insulin-like Growth Factor-Ⅱ Effects on the NSE Content of the Hippocampus after Ischemia/Reperfusion Injury Following Focal Cerebral Ischemia in Rat[J].Guangxi Sciences,2007,14(2):137-139. [点击复制]
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| 摘要: |
| 为了观察胰岛素样生长因子-Ⅱ(IGF-Ⅱ)对大鼠局灶性脑缺血再灌注不同时期海马皮层神经元特异性烯醇化酶(NSE)含量变化的影响,应用线栓法建立大鼠局灶性脑缺血再灌注损伤模型,将44只健康Spregue-Dawley(SD)大鼠随机分为假手术组(n=4)、缺血再灌注组和IGF-Ⅱ组。缺血再灌注组于脑缺血2h后根据再灌注不同时间(12h、24h、48h、72h、96h)分为5个亚组(每亚组4只);IGF-Ⅱ组于脑缺血2h侧脑室内注射IGF-Ⅱ 10μg后也根据再灌注不同时间(12h、24h、48h、72h、96h)分为5个亚组(每亚组4只)。于各时间点处死大鼠,并采用酶联免疫分析法测定每只鼠缺血侧海马皮层NSE含量。结果显示,缺血再灌注12h和24h组缺血侧海马皮层NSE含量较假手术组明显下降(P<0.01),48h和72h组也有明显下降,但(P<0.05),96h组基本恢复到假手术组水平;脑缺血2h侧脑室内注射IGF-Ⅱ 10μg后再灌注,48h和72h组与相应时间点缺血再灌注组比较明显上升(P<0.05),96h组基本恢复到假手术组水平。IGF-Ⅱ组NSE含量恢复比同时间点缺血再灌注组快,IGF-Ⅱ可能具有减轻缺血性脑损伤的作用。 |
| 关键词: 胰岛素样生长因子-Ⅱ 大鼠 脑缺血再灌注损伤 酶联免疫分析法 |
| DOI： |
| 投稿时间：2006-12-11 |
| 基金项目: |
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| Insulin-like Growth Factor-Ⅱ Effects on the NSE Content of the Hippocampus after Ischemia/Reperfusion Injury Following Focal Cerebral Ischemia in Rat |
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CHE Yu-qin1, SHEN Jing-lian1, DIAO Yao2, GAO Jie2
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| (1.The Fourth Affiliated Hospital, China Medical University, Liaoning, Shenyang, 110005, China;2.The First Affiliated Hospital, China Medical University, Liaoning, Shenyang, 110005, China) |
| Abstract: |
| To examine the influence of insulin-like growth factor-Ⅱ expression (IGF-Ⅱ) on the alteration of neuron-specific enolase (NSE) in the hippocampal neurons after ischemia/reperfusion following cerebral ischemia in rats. Method:Sprague-Dawley rats were subjected to 2 h of middle cerebral artery occlusion (MACO) by suture blockade and then reperfusion.44 rats were used in this study, they are sham rats (n=4), ischemia/reperfusion rats (n=20), and ischemia/reperfusion with IGF-Ⅱ treated rats (n=20).Ischemia/reperfusion and ischemia/reperfusion with IGF-Ⅱ treated rats were further divided into 5 sub-groups (4 rats each), 12h, 24h, 48h, 72h and 96h following reperfusion.IGF-Ⅱ treated rats were given IGF-Ⅱ 10μg by lateral ventricle injection at 2h post-MACO.The NSE content in hippocampus of ischemia side was examined by enzyme-linked immunosorbent assay. Results:so that, (1)The hippocampal NSE content was significantly reduced in ischemia/reperfusion injury rats compared to that in sham rats at 12h, 24h (P<0.01), 48h and 72h (P<0.05), the NSE content returned to the level of sham rats at 96h. (2) At 48h and 72h post-ischemia/reperfusion injury, the IGF-Ⅱ increased the NSE content in ischemia/reperfusion injured hippocampus (P<0.05) compared to those of ischemia/reperfusion injury without IGF-Ⅱ treatment. The NSE content returned to the level of sham rats at 96h after ischemia/reperfusion injury with IGF-Ⅱ treatment.Conclusion:IGF-Ⅱ treatment accelerates the NSE recovery in hippocampus after ischemia/reperfusion injury.The results suggest that the IGF-Ⅱ has a beneficial effect on ischemia/reperfusion injury after focal cerebral ischemia in rats. |
| Key words: insulin-like growth factor-Ⅱ rat ischemia/reperfusion injury enzymelinked immunosorbent assay |
