摘要: |
用橄榄胚作为橄榄组织培养的外植体,诱导胚长成无菌苗,截取成熟芽苗用于诱导侧芽及生根,筛选出MS+6 BA0.5+IBA0.5和MS+NAA1为侧芽诱导和生根培养的有效培养基,取得90%的侧芽诱导率和80%的生根率,为利用植物无性系快速繁殖技术繁育橄榄苗奠定基础。 |
关键词: 橄榄 胚 组织培养 诱导侧芽 生根 |
DOI: |
投稿时间:2004-08-04 |
基金项目: |
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Study of the Tissue Culture of Canarium album Raeusch |
Cai Hanquan
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(Biology Dept., Hanshan Teacher's College, Chaozhou, Guangdong, 521041, China) |
Abstract: |
The embryo of olive is used as explant in the tissue culture of olive,induced the embryo to grow up to be asepsis shoots,then cut the mature shoots for the inducement of lateral bud and radication,screened out MS+6-BA0.5 and MS+NAA1 as the effective substrate of lateral bud abduction and radication cultivating,and acquired lateral bud abduction rate which was up to 90% and radication rate 80%.This established the base of using plant clone to propagate technic-breeding olive seedling rapidly.This kind of method is useful for some kind of woody plant which is difficult to be cultivated and some economic plants that contain higher alkaloids.It is a tissue culture example of this kind of plant. |
Key words: olive embryo tissue culture sprouting of lateral bud rooting |