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刘玉,杨键,李茹,龙丽娟.微生物Rieske型芳香环双加氧酶研究进展[J].广西科学院学报,2020,36(3):272-281. [点击复制]
- LIU Yu,YANG Jian,LI Ru,LONG Lijuan.Research Advances of Microbial Rieske-type Aromatic Ring Dioxygenases[J].Journal of Guangxi Academy of Sciences,2020,36(3):272-281. [点击复制]
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| 微生物Rieske型芳香环双加氧酶研究进展 |
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刘玉1,2, 杨键1,3, 李茹1,2, 龙丽娟1,2,3
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| (1.中国科学院南海海洋研究所, 中国科学院热带海洋生物资源与生态重点实验室, 广东广州 510301;2.中国科学院大学, 北京 100049;3.南方海洋科学与工程广东省实验室(广州), 广东广州 511458) |
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| 摘要: |
| Rieske型芳香环双加氧酶是含有Rieske[2Fe-2S]中心的多组分酶系统，能够催化多种反应，在有机污染物生物降解和化学品绿色合成领域具有广阔的开发应用前景。Rieske型芳香环双加氧酶依靠还原酶、铁硫还原蛋白及末端氧化酶的铁硫中心进行电子传递，利用氧气实现芳香烃类化合物的立体选择性羟化双加氧反应。该类酶具有广泛的底物谱、高度立体选择性等特点，在芳香族污染物降解、环境微生物修复以及手性化合物合成等方面发挥重要作用，是现代生物催化理论与应用热点研究对象之一。本文总结了微生物Rieske型芳香环双加氧酶的结构组成、催化机制和实际应用等方面的研究进展，以期为相关研究工作提供参考资料。 |
| 关键词: Rieske型芳香环双加氧酶 分类 晶体结构 催化机制 蛋白质工程 应用 |
| DOI：10.13657/j.cnki.gxkxyxb.20201027.015 |
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| 基金项目:中科院战略性先导科技专项（XDA13020301），广东省基础与应用基础研究基金项目（2019A1515011629），广州市科技计划项目（201904010165），南方海洋科学与工程广东省实验室（广州）人才团队引进重大专项（GML2019ZD0404）和广东海洋经济创新发展区域示范项目（GD2012-D01-002）资助。 |
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| Research Advances of Microbial Rieske-type Aromatic Ring Dioxygenases |
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LIU Yu1,2, YANG Jian1,3, LI Ru1,2, LONG Lijuan1,2,3
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| (1.CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, Guangdong, 510301, China;2.University of Chinese Academy of Sciences, Beijing, 100049, China;3.Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou), Guangzhou, Guangdong, 511458, China) |
| Abstract: |
| Rieske-type aromatic ring dioxygenases are multi-component enzyme systems containing the Rieske [2Fe-2S] center, which can catalyze a variety of chemical reactions. They have broad development and application prospects in the field of biodegradation of organic pollutants and green synthesis of chemicals.Rieske-type aromatic ring dioxygenases rely on the iron-sulfur center of reductase, iron-sulfur of ferredoxin and terminal oxygenase for electron transfer, and realize stereoselective hydroxylation of aromatic hydrocarbon compounds by oxygen. This type of enzyme has the characteristics of a wide substrate spectrum and high stereoselectivity. It plays significant roles in the degradation of aromatic pollutants, the remediation of environmental microorganisms and synthesis of chiral compounds. It is one of the hot research objects in the theory and application of modern biocatalysis. In this article, the research progress of the structural composition, catalytic mechanism and application of Rieske-type aromatic ring dioxygenases were reviewed to provide reference for related research work. |
| Key words: Rieske-type aromatic ring dioxygenases classification crystal structure catalytic mechanism protein engineering application |
