| 引用本文： |
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王桂文,黄庶识,陶站华.基于拉曼镊子的发酵过程单细胞分析：应用与展望[J].广西科学院学报,2020,36(3):260-271. [点击复制]
- WANG Guiwen,HUANG Shushi,TAO Zhanhua.Single-cell Analysis of Microbial Fermentation Based on Raman Tweezers:Application and Prospect[J].Journal of Guangxi Academy of Sciences,2020,36(3):260-271. [点击复制]
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| 摘要: |
| 微生物发酵是个复杂的过程，而细胞间的异质性对微生物发酵性能有重要的影响。通过单细胞分析了解微生物细胞的异质性，利于发酵环境的优化，从而减少细胞间的异质性，提高发酵性能。整合显微拉曼光谱和光学微操控的拉曼镊子是实时、无损、无须标记且快速灵敏的单细胞分析方法。本文重点回顾拉曼镊子在聚酯、类胡萝卜素、脂肪酸、生物乙醇以及重组蛋白等发酵生产过程中的应用，并分析其在应用上存在的不足。在克服自身不足、融合新的分析技术与数据处理方法后，拉曼镊子将是了解细胞异质性、认知复杂生物过程的有力手段。 |
| 关键词: 微生物发酵 拉曼光谱 拉曼镊子 异质性 单细胞分析 |
| DOI：10.13657/j.cnki.gxkxyxb.20201027.010 |
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| 基金项目:国家自然科学基金项目（30760010，31060128）和广西自然科学基金项目（2017GXNSFAA198029，2013GXNSFAA019043，2012GXNSFCA053001，桂科自0832022Z，桂科自0991079）资助。 |
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| Single-cell Analysis of Microbial Fermentation Based on Raman Tweezers:Application and Prospect |
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WANG Guiwen, HUANG Shushi, TAO Zhanhua
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| (Guangxi Academy of Sciences, Nanning, Guangxi, 530007, China) |
| Abstract: |
| Microbial fermentation is a complex processing,and the heterogeneity between cells has a significant impact on the performance of microbial fermentation.Understanding the heterogeneity of microbial cells through single-cell analysis is beneficial to the optimization of the fermentation environment,thereby reducing the heterogeneity between cells and improving the performance of fermentation. Raman tweezers,a technique that integrates Raman micro-spectroscopy and optical micromanipulation,is a real-time,non-destructive,label-free,fast and sensitive single-cell analysis method.This article focuses on reviewing the application of Raman tweezers in the fermentation process of polyester,carotenoids,fatty acids,bio-ethanol and recombinant proteins,and analyzes the deficiencies in the application.After conquering its own shortcomings and integrating new analytical techniques and data processing methods,Raman tweezers is expected to be a more powerful method to reveal the cell heterogeneity and to recognize the complex biological processes. |
| Key words: microbial fermentation Raman spectroscopy Raman tweezers heterogeneity single-cell analysis |
