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  • 梁莹,李林轩,蔡锦源,林伟,秦双双,张重义,缪剑华,李蕴琦,韦坤华.金线莲种质的生理生化特性及内源激素含量差异[J].广西科学学院学报,2019,35(1):45-50.    [点击复制]
  • LIANG Ying,LI Linxuan,CAI Jinyuan,LIN Wei,QIN Shuangshuang,ZHANG Zhongyi,MIAO Jianhua,LI Yunqi,WEI Kunhua.Differences of Physiological and Biochemical Activities and Endogenous Hormone Contents in Anoectochilus roxburghii Germplasms[J].Journal of Guangxi Academy of Sciences,2019,35(1):45-50.   [点击复制]
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金线莲种质的生理生化特性及内源激素含量差异
梁莹1,2, 李林轩1, 蔡锦源3, 林伟1, 秦双双1,2, 张重义2, 缪剑华1, 李蕴琦3, 韦坤华1
0
(1.广西药用植物园, 广西药用资源保护与遗传改良重点实验室, 广西南宁 530023;2.福建农林大学作物科学学院, 福建福州 350002;3.广西科技大学鹿山学院, 广西柳州 545616)
摘要:
比较两个金线莲Anoectochilus roxburghii(Wall.)Lindl.种质的生理生化特性及内源激素含量差异,为后续的金线莲优良品种选育提供参考依据。本研究以福建小圆叶金线莲和福建黑叶金线莲30 d扩繁丛生苗、30 d生根试管苗和30 d温室移栽苗为材料,采用酶联免疫吸附测定法(ELISA)分别测定两个金线莲种质的内源激素含量;可溶性蛋白、可溶性糖、叶绿素和丙二醛(MDA)含量,以及过氧化物酶(POD)活性采用分光光度法测定。试验结果显示在扩繁、生根、移栽过程中,福建小圆叶金线莲可溶性蛋白含量、可溶性总糖含量、POD活性、MDA含量及其体内脱落酸(ABA)、赤霉素(GA)含量均高于福建黑叶金线莲,且两个不同种质金线莲扩繁、生根苗的可溶性蛋白、可溶性总糖及其体内玉米素(ZR)、GA和生长素(IAA)含量均显著(P<0.05)高于移栽苗。因此,福建小圆叶金线莲可能更易于适应环境的变化。
关键词:  金线莲  生理生化特性  内源激素含量
DOI:10.13657/j.cnki.gxkxyxb.20190123.005
基金项目:国家中医药行业科研专项(201507002),国家中药材产业技术体系项目(CARS-21),广西科技计划项目(桂科AD17129044),广西药用资源保护与遗传改良重点实验室开放基金项目(2017),广西医疗卫生适宜技术研究与开发项目(S201530)和河池市科学研究与技术开发计划项目(河科攻1489-2-20)资助。
Differences of Physiological and Biochemical Activities and Endogenous Hormone Contents in Anoectochilus roxburghii Germplasms
LIANG Ying1,2, LI Linxuan1, CAI Jinyuan3, LIN Wei1, QIN Shuangshuang1,2, ZHANG Zhongyi2, MIAO Jianhua1, LI Yunqi3, WEI Kunhua1
(1.Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement, Guangxi Botanical Garden of Medicinal Plants, Nanning, Guangxi, 530023, China;2.College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China;3.Lushan College of Guangxi University of Science and Technology, Liuzhou, Guangxi, 545616, China)
Abstract:
The physiological and biochemical characteristics and endogenous hormone content of two germplasms of Anoectochilus roxburghii were compared to provide reference for the subsequent breeding of fine varieties of A.roxburghii. The 30-day of propagation cluster seedlings, 30-day of rooting tube seedlings and 30-day of greenhouse transplanting seedlings of Fujian A.roxburghii with small round leaves and Fujian A.roxburghii with black leaves were used as materials. The content of endogenous hormones in two germplasms of A.roxburghii was determined by enzyme-linked immunosorbent assay (ELISA). The contents of soluble protein, soluble sugar, chlorophyll, malondialdehyde (MDA) and the activity of Peroxidase (POD) in two germplasms of A.roxburghii were measured by spectrophotometry. The results showed that during the process of propagation, rooting and transplanting, the contents of soluble protein, total soluble sugar, MDA, ABA, GA and the activity of POD in A. roxburghii with small round leaves were higher than those in A.roxburghii with black leaves. Moreover, in the process of propagation and rooting, the contents of soluble protein, total soluble sugar, ZR, GA and IAA in two germplasms of A.roxburghii were significantly (P<0.05) higher than those of transplanting seedlings. Therefore, A.roxburghii with small round leaves may be easier to adapt to changes in the environment.
Key words:  Anoectochilus roxburghii (Wall.) Lindl  physiological and biochemical activities  endogenous hormone content

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