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  • 辛美丽,宋爱环,王志刚,胡凡光,逄劭楠,孙福新.芽孢杆菌组合对海水养殖水体COD的降解效果[J].广西科学院学报,2016,32(3):215-220.    [点击复制]
  • XIN Meili,SONG Aihuan,WANG Zhigang,HU Fanguang,PANG Shaonan,SUN Fuxin.Study on COD Degradation Capability of Bacillus Combinations[J].Journal of Guangxi Academy of Sciences,2016,32(3):215-220.   [点击复制]
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芽孢杆菌组合对海水养殖水体COD的降解效果
辛美丽, 宋爱环, 王志刚, 胡凡光, 逄劭楠, 孙福新
0
(山东省海洋生物研究院, 山东青岛 266104)
摘要:
[目的]获得一种降解性能优于单菌株的菌株组合,更好地改善目前工业化密度养殖的水体水质。[方法]从刺参(Stichopus japonicus)养殖池塘分离降解效果良好的单菌株,根据菌株之间的拮抗效应,按照2株菌或3株菌进行组合,研究各组合菌株的降解效果。[结果]2株菌的组合第6天时COD含量的均值为422.57 mg/L,标准差为63.85,3株菌的组合第6天时COD含量的均值为365.61 mg/L,标准差为67.63,说明3株菌的组合整体降解效果比2株菌的组合好,且更容易获得降解效率高的组合,但并不是所有3株菌的组合降解效果都优于2株菌的组合。[结论]确定最优菌株组合为C14(菌株B2,菌株B6,菌株B11),COD降解率为64.29%。经鉴定,菌株B2为枯草芽孢杆菌(Bacillus subtiliss),菌株B6为巨大芽孢杆菌(Bacillus megaterium),菌株B11为嗜碱性芽孢杆菌(Bacillus alcalophilus),为今后的实践应用提供参考。
关键词:  芽孢杆菌  降解性能  组合
DOI:10.13657/j.cnki.gxkxyxb.20160826.008
投稿时间:2016-04-10
基金项目:海洋公益性行业科研专项经费项目(201305005)和海洋经济创新发展区域示范项目资助。
Study on COD Degradation Capability of Bacillus Combinations
XIN Meili, SONG Aihuan, WANG Zhigang, HU Fanguang, PANG Shaonan, SUN Fuxin
(Marine Biology Institute of Shandong Province, Qingdao, Shandong, 266104, China)
Abstract:
[Objective] To improve the water quality in high stocking density of industrial aquaculture,a kind of strain combination,with degradation capability better than single strain, was found.[Methods] Several bacterial strains with good degradation property were separated from Stichopus japonicus culture ponds. Antagonistic effect between strains was studied. According to the results of the antagonistic effect, the degradation capability of COD of the combinations of 2 or 3 strains were studied.[Results] The mean COD of the combinations of two strains at the 6th day was 422.57 mg/mL,and standard deviation was 63.85. The mean COD of the combinations of three strains at the 6th day was 365.61 g/mL and the standard deviation was 67.63. The results showed that combinations of three strains compared to combinations of two strains were easier to get a combination with better degradation efficiency.[Conclusion] Finally,the optimal combination was C14(strain B2,strain B6,strain B11) with COD degradation rate of 64.29%. The strain B2, strain B6 and strain B11 were separately identified as Bacillus subtiliss,Bacillus megaterium and Bacillus alcalophilus. The result was a reference for future applications of strains.
Key words:  Bacillus  degradation capability  combination

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