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刘莹,刘名,于超勇,宋静静,官曙光,徐涛,于道德.外源核苷酸对大菱鲆幼鱼生长和溶菌酶活力的影响[J].广西科学院学报,2016,32(2):116-121. [点击复制]
- LIU Ying,LIU Ming,YU Chaoyong,SONG Jingjing,GUAN Shu-guang,XU Tao,YU Daode.Effect of a Nucleotide-enriched Diet on the Growth and Lysozyme Activity in Juvenile Turbot (Scophthalmus maximus)[J].Journal of Guangxi Academy of Sciences,2016,32(2):116-121. [点击复制]
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| 外源核苷酸对大菱鲆幼鱼生长和溶菌酶活力的影响 |
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刘莹1,2, 刘名1,2, 于超勇1,2, 宋静静1,2, 官曙光1,2, 徐涛3, 于道德1,2
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| (1.山东省海洋生物研究院, 山东青岛 266104;2.山东省海水养殖工程技术研究中心, 山东青岛 266104;3.山东省渔业技术推广站, 山东济南 250013) |
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| 摘要: |
| [目的]研究外源核苷酸对大菱鲆(Scophthalmus maximus)幼鱼生长和免疫力的影响,为核苷酸营养在大菱鲆养殖上的推广应用提供依据。[方法]以鱼粉为主要蛋白源,在基础饲料中分别添加不同含量的核苷酸,添加量(W/W)分别为0.00%,0.01%,0.03%,0.10%,0.30%,0.50%。在人工养殖的情况下(自然光照,水温11.0~16.3℃),对初始体质量为12.07~12.29 g的大菱鲆幼鱼进行核苷酸营养的外源添加实验,分别在4周和8周对不同组别的生长和生态转化效率进行定期测定。然后在8周实验结束时,对大菱鲆血清中溶菌酶活力进行测定。[结果]随着在基础饲料中核苷酸添加量的增加,大菱鲆的特定生长率逐渐减小,且大菱鲆生态转化效率实验组小于对照组,而血清中溶菌酶活性实验组高于对照组。[结论]核苷酸对大菱鲆幼鱼的生长无明显的促进或抑制作用,对血清中的溶菌酶活性具有促进作用。 |
| 关键词: 核苷酸 大菱鲆幼鱼 生长 溶菌酶 |
| DOI： |
| 投稿时间：2016-03-29修订日期：2016-05-09 |
| 基金项目:山东省农业良种工程"优质抗病速生鱼类良种选育——速生耐温工厂化适养品种-大菱鲆选育"和山东省现代农业产业体系鱼类创新团队项目(SDAIT-14-011-04)资助。 |
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| Effect of a Nucleotide-enriched Diet on the Growth and Lysozyme Activity in Juvenile Turbot (Scophthalmus maximus) |
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LIU Ying1,2, LIU Ming1,2, YU Chaoyong1,2, SONG Jingjing1,2, GUAN Shu-guang1,2, XU Tao3, YU Daode1,2
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| (1.Marine Biology Institute of Shandong Province, Qingdao, Shandong, 266104, China;2.Mariculture Engineering Research Center of Shandong Province, Qingdao, Shandong, 266104, China;3.Shandong Province Fisheries Technology Extension Station, Jinan, Shandong, 250013, China) |
| Abstract: |
| [Objective] The research of exogenous nucleotides (EN) effecting on growth and innate immunity of juvenile turbort (Scophthalmus maximus),which provides the evidence for the popularization and application of Scophthalmus cultivation.[Methods] With a basal diet formulated from fish meal containing 47% crude protein and 1 1% lipid by adding exogenous nucleotides of 0.00%,0.01%,0.03%, 0.10%, 0.30%,0.50%,respectively (W/W). Each diet was distributed by hand to triplicate groups of 15 fish and the growth trial lasted 8 weeks.[Results] At the end of 4 weeks and 8 weeks,the special growth rate and ecological conversion efficiencies were the same for all groups. Although a reverse relationship was found between the special growth rate and EN levels, also, a weaker relationship was found between the ecological conversion efficiencies and EN levels. The activity of lysosomal enzymes is promoting.[Conclusion] We conclude that, EN is not having positive or negative on growth rate of S. maximus,at least at the stage of juvenile. However, elevated activity of lysosomal enzymes in blood serum indicates its promotion of innate immunity. |
| Key words: nucleotide juvenile turbot (Scophthalmus maximus) growth lysozyme |
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