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  • 滕红丽,郭力城,赵湘培.薄层色谱法评价不同产地解毒草的品质[J].广西科学院学报,2015,31(4):302-304.    [点击复制]
  • TENG Hong-li,GUO Li-cheng,ZHAO Xiang-pei.Identification of Onychiumjaponicum (Thunb.) kunze var.Japonicum from Different Origin by TLC[J].Journal of Guangxi Academy of Sciences,2015,31(4):302-304.   [点击复制]
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薄层色谱法评价不同产地解毒草的品质
滕红丽, 郭力城, 赵湘培
0
(广西壮族自治区民族医药研究院/广西壮医医院, 广西南宁 530001)
摘要:
[目的]建立评价解毒草(Onychiumjaponicum(Thunb.) kunze var.Japonicum)薄层色谱分析方法。[方法]以有效成分菊苣酸为指标,以石油醚-乙酸乙酯-甲酸(3:6:1)为展开剂,建立解毒草的专属性薄层色谱,并评价10个不同产地解毒草的品质。[结果]不同产地解毒草药材与对照色谱在相应位置上,显相同颜色的斑点,且斑点清晰,比移值(Rf)为0.66。[结论]薄层色谱法能够有效对解毒草进行鉴别,且该方法简便、准确,具有较好的适应性。
关键词:  解毒草  菊苣酸  薄层色谱
DOI:
投稿时间:2015-04-10
基金项目:国家科技支撑计划项目(2012BAI27B06-1,2013BAI11B06-6),广西重大专项计划项目(桂科重14124002-14)和南宁市科技攻关与新产品试制项目(20133158,20133163)资助。
Identification of Onychiumjaponicum (Thunb.) kunze var.Japonicum from Different Origin by TLC
TENG Hong-li, GUO Li-cheng, ZHAO Xiang-pei
(Guangxi Institute of Minority Medicine/Traditional Zhuang Hospital of Guangxi, Nanning, Guangxi, 530001, China)
Abstract:
[Objective] To establish a TLC method for the identification of Onychiumjaponicum (Thunb.) kunze var.japonicum.[Methods] Chicoric acid used as indicators and petroleum ether-ethyl acetate-formic acid(3:6:1) used as developing agent.[Results] The same color spots were shown in the corresponding position of the Onychiumjaponicum (Thunb.) kunze var.japonicum from different origin,and the spots were clear.The Rf index was 0.66.[Conclusion] The TLC method is effective for the identification of the Onychiumjaponicum (Thunb.) kunze var.japonicum, and it is simple and reliable.
Key words:  Onychiumjaponicum(Thunb.) kunze var.japonicum  chicoric  TLC

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