| 引用本文： |
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郑元涛,欧娜,韦星明.水稻转录因子WRKY39的克隆表达及DNA结合活性分析[J].广西科学院学报,2012,28(3):197-200. [点击复制]
- ZHENG Yuan-tao,OU Na,WEI Xing-ming.Cloning and Expression of a Rice WRKY39 Gene and DNA-binding Analysis[J].Journal of Guangxi Academy of Sciences,2012,28(3):197-200. [点击复制]
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| 摘要: |
| 根据水稻转录因子WRKY39的cDNA基因序列设计引物,克隆水稻日本晴(Oryza sativa L.)WRKY类转录因子的cDNA基因并进行原核表达,通过凝胶阻滞实验分析融合蛋白与DNA的结合活性。结果克隆了1个新的WRKY基因OsWRKY39,其编码的蛋白OsWRKY39能够与顺式元件W盒特异结合,OsWRKY39具有转录因子的最基本特征。 |
| 关键词: 转录因子 WRKY 水稻 原核表达 DNA结合活性 |
| DOI： |
| 投稿时间：2012-03-12修订日期：2012-06-06 |
| 基金项目: |
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| Cloning and Expression of a Rice WRKY39 Gene and DNA-binding Analysis |
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ZHENG Yuan-tao, OU Na, WEI Xing-ming
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| (Biology Institute of Guangxi Academy of Science, Nanning, Guangxi, 530007, China) |
| Abstract: |
| A new rice WRKY gene (OsWRKY39) was cloned from cDNA of young rice leaves employing a PCR strategy and was expressed in E.coli (BL 21 strain).The DNA-binding activity of the recombinant WRKY39 protein was analyzed using electrophoretic mobility shift assay. It was found that the recombinant WRKY39 protein bound specifically to W-box, which indicated WRKY39 was a transcriptional factor. |
| Key words: transcriptional factor WRKY Oryza sativa prokaryotic expression DNA-binding activity |
