| 引用本文： |
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覃葆,江海燕,蔡少芳,付晓,邓欢,史勤怡.不同产地广山楂中金丝桃苷含量比较[J].广西科学院学报,2011,27(3):196-198. [点击复制]
- QIN Bao,JIANG Hai-yan,CAI Shao-fang,FU Xiao,DENG Huan,SHI Qin-yi.Comparison the Content of Hyperoside of Malus Doumeri(Bois) Chev. from Different Producing Area[J].Journal of Guangxi Academy of Sciences,2011,27(3):196-198. [点击复制]
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| 摘要: |
| 采用高效液相色谱法测定不同产地的广山楂中金丝桃苷的含量。色谱柱:C18-ODS、流动相:乙腈-0.1%磷酸水溶液(12:88)、流速:1ml/min、检测波长:360nm、柱温:30℃。结果表明:金丝桃苷在进样量为0.032~1.28μg的范围内呈良好的线性关系(r=0.9999),平均回收率为98.9%,RSD值为2.22%。不同产地广山楂金丝桃苷含量存在一定差异,其中贺州产广山楂金丝桃苷含量最高。 |
| 关键词: 广山楂 金丝桃苷 含量比较 |
| DOI： |
| 投稿时间：2011-05-25 |
| 基金项目:广西科技攻关项目(桂科攻0815005-1-9)资助 |
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| Comparison the Content of Hyperoside of Malus Doumeri(Bois) Chev. from Different Producing Area |
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QIN Bao, JIANG Hai-yan, CAI Shao-fang, FU Xiao, DENG Huan, SHI Qin-yi
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| (Guangxi Traditional Chinese Medical University, Nanning, Guangxi, 530001, China) |
| Abstract: |
| The content of hyperoside of Malus doumeri(Bois) Chev.from different producing area was determined by HPLC.The C18 ODS was used at the temperature of 30℃.The mobile phase consisted of acetonitrile and 0.1% orthophosphoric acid(12:88) with isocratic elution.The UV detector was set at 360nm with 1.0ml/min flow rate.Results showed that a new standard curve,y=1832.3x+15.938 and r=0.9999,was established.When the hyperoside sample size was between 0.032~1.28μg,the standard curve showed a good linear relationship with 98.9% average recovery and 2.22% RSD.According to the results from HPLC,the content of hyperoside of Malus doumeri(Bois) Chev.was various from different producing area.The highest content of hyperoside was found in Malus doumeri(Bois) Chev.produced in Hezhou. |
| Key words: Malus doumeri(Bois) Chev. hyperoside content comparison |
