| 引用本文： |
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王晔,彭元,赵肃清,徐凤彩.乙酰胆碱酯酶膜的酶学性质及其对有机磷农药的检测效果[J].广西科学院学报,2007,23(3):160-162. [点击复制]
- WANG Ye,PENG Yuan,ZHAO Su-qing,XU Feng-cai.The Nature of Acetylcholinesterase Membrane and Its Detection Effects on Organophosphorus Pesticides[J].Journal of Guangxi Academy of Sciences,2007,23(3):160-162. [点击复制]
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| 摘要: |
| 测定自行制备的乙酰胆碱酯酶(AChE)酶液和壳聚糖-AChE酶膜的酶学性质,并以壳聚糖-AChE酶膜分别检测乐果、甲胺磷、敌百虫、敌敌畏、甲基对硫磷5种有机磷农药,探讨采取酶膜进行快速检测有机磷农药残留的可行性。结果显示:壳聚糖-AChE酶膜的KMapp为2.8×10-4 mol/L,最适pH值为8.0,在pH值为8.0~8.5时活力稳定;最适反应温度为45℃左右,在15~60℃时较为稳定;半衰期达12d,固定化酶膜的实际应用性能较游离酶有较大改善。壳聚糖-AChE酶膜对不同类型的有机磷农药的敏感性差异很大,对甲基对硫磷最为敏感,其pI50为5.57,对其它4种农药pI50为4.0左右;以酶活力抑制率16%为农药检出标准,甲基对硫磷的检出限为0.4m g/L,其他4种检出限为3~22m g/L,只有甲基对硫磷达到检测要求。 |
| 关键词: 乙酰胆碱酯酶 壳聚糖 固定化酶 酶学性质 有机磷农药 检测 |
| DOI： |
| 投稿时间：2007-03-07修订日期：2007-04-25 |
| 基金项目:广西区科技厅攻关项目(桂科攻0537007-7-2);南宁市科技攻关项目(200501065B)资助 |
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| The Nature of Acetylcholinesterase Membrane and Its Detection Effects on Organophosphorus Pesticides |
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WANG Ye1,2, PENG Yuan2, ZHAO Su-qing3, XU Feng-cai4
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| (1.Sinoasis Pharmaceuticals Inc, Guangzhou, Guangdong, 510760, China;2.Guangxi Academy of Sciences, Nanning, Guangxi, 530007, China;3.Guangxi Institute of Botany, Guilin, Guangxi, 541006, China;4.College of Life Science, South China Agricultural University, Guangzhou, Guangdong, 510642, China) |
| Abstract: |
| In this article,Chitosan AChE membrane of Acetylcholinesterase and the nature of the AChE membrane is determined,and by using this AChE membrane,five kinds of organophosphrous pesticides(methyl parathion,dimethoate,dichlorovos,trichlorfon and methamidophos) are detected.The result shows that the kapp M of immobilized AChE is 2.8×10-4 mol/L.The optimum pH value is 8.0,while the pH value stability is 8.0~8.5.The thermal stability of the immobilized AChE is 15~60℃ and the operation half life of the immobilized AChE is 12 days.The practical performance of the immobilized AChE is greatly improved.Immobilized Acetylcholinesterase's sensitivity changes remarkably depending on the kinds of organophosphrous.It is most sensitive to methyl parathion whose pI50 is 5.57.Yet,it is less sensitive to the other four kinds of pesticides whose pI50 is about 4.0.The detection limit to methyl parathion is 0.4mg/L,while detection limit to the others is between 3~22 mg/L. |
| Key words: Acetylcholinesterase Chitosan immobilized enzyme zymological properties organophosphrous pesticide detection |
