| 引用本文： |
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黎瑞,赵翠红,闫莲姣,苏桂前,陆巧燕.防芷鼻炎片质量标准研究[J].广西科学院学报,2006,22(S1):471-474. [点击复制]
- LI Rui,ZHAO Cui-hong,YAN Lian-jiao,SU Gui-qian,LU Qiao-yan.Study on Quality Standard for Fangzhibiyan Tablets[J].Journal of Guangxi Academy of Sciences,2006,22(S1):471-474. [点击复制]
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| 摘要: |
| 采用薄层色谱法对防芷鼻炎片中的野菊花、防风、胆南星及蒺藜进行定性鉴别,并采用高效液相色谱法(HPLC)测定白芍中芍药苷的含量。HPLC的色谱柱为C18柱(4.6mm×250mm,5μm),流动相为乙腈-0.05mol/L磷酸二氢钾溶液(15:85),检测波长为230nm。鉴别项下薄层图斑点清晰,阴性对照无干扰,专属性强。芍药苷含量测定在进样浓度10.4~52.0μg/ml之间呈良好的线性关系,r=0.9998,回收率为98.73%,RSD=1.93%。本方法简便准确,重现性好,可以用于防芷鼻炎片的质量标准。 |
| 关键词: 薄层色谱法 高效液相色谱法 芍药苷 防芷鼻炎片 |
| DOI： |
| 投稿时间：2006-11-06 |
| 基金项目: |
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| Study on Quality Standard for Fangzhibiyan Tablets |
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LI Rui, ZHAO Cui-hong, YAN Lian-jiao, SU Gui-qian, LU Qiao-yan
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| (Nanning Dizhi Pharmaceutical Co. Ltd., Nanning, Guangxi, 530003, China) |
| Abstract: |
| Flos Chrysanthemi indici,Radix Saposhnikoviae,arisaema cum bile,caltrop were identified quantitatively by TLC.Paeoniflorin content in Cortex Phellodendri Chinensis determined by HPLC.HPLC was applied with a C18 column,and the mobile phase of methy1 cyanide-0.05mol/L potassium dihydrogen phosphate(15:85),and the detection wavelength was 230nm.The TLC chromatogram spots were clear and no inteference was found in the negative reference.Paeoniflorin was obtained in the range of 10.4~52.0μg/ml,r=0.9998.The average recovery of Paeoniflorin was 98.73%,RSD=1.93%.This method is simple,accurate and has good reproducibility,which can be used for a quality control standard of Fangzhibiyan tablets. |
| Key words: TLC HPLC Paeoniflorin fangzhibiyan tablets |
