| 引用本文： |
-
黄岛平,陈秋虹,劳燕文.柱前衍生高效液相色谱法测定红牛维生素功能饮料中牛磺酸含量[J].广西科学院学报,2006,22(S1):419-421. [点击复制]
- HUANG Dao-ping,CHEN Qiu-hong,LAO Yan-wen.Taurine in RedBull Super Vitamin Drinks Determined by Pre-column Derivatization and HPLC[J].Journal of Guangxi Academy of Sciences,2006,22(S1):419-421. [点击复制]
|
|
| 摘要: |
| 用邻苯二甲醛(OPA)柱前衍生高效液相色谱法,运用C18色谱柱为固定相,甲醇+0.05mol/L NaAc溶液(45+55)为流动相,检测波长330nm,测定红牛维生素功能饮料的牛磺酸。结果衍生化反应迅速,分离效果好,在10~50μg/ml的浓度范围内,牛磺酸色谱峰面积与浓度之间的线性相关系数为0.9999,加标回收率为97.48%~101.0%,RSD为2.5%。 |
| 关键词: 高效液相色谱法 牛磺酸 饮料 |
| DOI： |
| 投稿时间：2006-08-20 |
| 基金项目: |
|
| Taurine in RedBull Super Vitamin Drinks Determined by Pre-column Derivatization and HPLC |
|
HUANG Dao-ping, CHEN Qiu-hong, LAO Yan-wen
|
| (Guangxi Research Center for Analysis and Test, Nanning, Guangxi, 530022, China) |
| Abstract: |
| Taurine in RedBull Super Vitamin Drinks was determined by OPA pre-column derivatization and High Performance Liquid Chromatography(HPLC),using C18 column as fixed phase,methyl alcohol+0.05mol/L NaAc as mobile phase(45+55 by volume),with wavelength 330nm.The separation effect is satisfactory;derivatization reaction is rapid;the linear correlation coefficients of peak areas and concentration is 0.9999 in the range of 1050g/ml respectively;the recoveries of the Taurine were 97.48%~101.0%,RSD were 2.5%.This method is accurate and suitable for quality control of Tongluoxiaru Koufuye. |
| Key words: HPLC taurine drinks |
