| 引用本文： |
-
马兰珍,韦立秀,薛鹰,黄宝灵,吕成群.杂交松组织培养中外植体的灭菌方法[J].广西科学院学报,2005,(1):37-39. [点击复制]
- Ma Lanzhen,Wei Lixiu,Xue Ying,Huang Baoling,Lü Chengqun.Explant Disinfection in Tissue Culture of Pinus elliottii×P. caribaea[J].Journal of Guangxi Academy of Sciences,2005,(1):37-39. [点击复制]
|
|
| 摘要: |
| 在实验室内对来自广西大学林学院苗圃、东门林场苗圃、广西林业局种苗基地的杂交松(Pinus elliottii×P.caribaea)外植体进行75%酒精10s+0.1%升汞4~6 min灭菌试验。其中,东门林场苗圃的外植体进一步采用75%酒精10s+0.1% HgCl2 6min结合添加入培养基中的1.2%大蒜液的灭菌方法进行试验。结果表明:广西大学林学苗圃的杂交松外植体,采用75%酒精10s+0.1%升汞5~6 min的灭菌方法效果较好。东门林场苗圃以及广西林业局种苗基地的杂交松外植体用75%酒精+0.1%升汞的灭菌方法效果不理想。东门林场苗圃的枝条用75%酒精10s+0.1% HgCl2 6min结合添加入培养基中的1.2%大蒜液的灭菌方法效果较好。 |
| 关键词: 杂交松 组织培养 外植体 消毒灭菌 |
| DOI： |
| 投稿时间：2004-06-29修订日期：2004-08-09 |
| 基金项目:广西科学研究与技术开发计划(桂科攻0235017-6)资助项目。 |
|
| Explant Disinfection in Tissue Culture of Pinus elliottii×P. caribaea |
|
Ma Lanzhen, Wei Lixiu, Xue Ying, Huang Baoling, Lü Chengqun
|
| (Forestry College, Guangxi University, Nanning, Guangxi, 530001, China) |
| Abstract: |
| The disinfection experiments of 75% ethanol 10 seconds+0.1% HgCl2 4~6 minutes to Pinus elliottii×P.caribaea explants from the nurseries of Forestry College(FC),Dongmen Forest Farm(DFF) and Guangxi Forestry Bureau (GFB)were performed in lab.To the explants from DFF,a further disinfection experiment was conducted in the treatment of 75% ethanol 10 seconds+0.1% HgCl2 6 minutes with 1.2% garlic solution in the culture medium.It shows that the treatment of 75% ethanol 10 seconds+0.1% HgCl2 5~6 minutes has better result to the explants from FC.The treatment of 75% ethanol+0.1% HgCl2 is no good to the explants from DFF and GFB.To the explants from DFF,there is a better result in the treatment of 75% ethanol 10 seconds+0.1% HgCl2 6 minutes with 1.2% garlic solution in the culture medium. |
| Key words: Pinus elliottii×P.caribaea tissue culture explants disinfection |
